State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Veterinary Public Health of Agriculture Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, PR China.
PLoS One. 2013 Aug 22;8(8):e71300. doi: 10.1371/journal.pone.0071300. eCollection 2013.
We determined the complete mitochondrial DNA (mtDNA) sequence of a fluke, Paramphistomum cervi (Digenea: Paramphistomidae). This genome (14,014 bp) is slightly larger than that of Clonorchis sinensis (13,875 bp), but smaller than those of other digenean species. The mt genome of P. cervi contains 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (NCRs), a complement consistent with those of other digeneans. The arrangement of protein-coding and ribosomal RNA genes in the P. cervi mitochondrial genome is identical to that of other digeneans except for a group of Schistosoma species that exhibit a derived arrangement. The positions of some transfer RNA genes differ. Bayesian phylogenetic analyses, based on concatenated nucleotide sequences and amino-acid sequences of the 12 protein-coding genes, placed P. cervi within the Order Plagiorchiida, but relationships depicted within that order were not quite as expected from previous studies. The complete mtDNA sequence of P. cervi provides important genetic markers for diagnostics, ecological and evolutionary studies of digeneans.
我们确定了鹿平腹吸虫（双腔科）的完整线粒体 DNA（mtDNA）序列。该基因组（14014bp）略大于华支睾吸虫（13875bp），但小于其他吸虫的基因组。鹿平腹吸虫的 mt 基因组包含 12 个蛋白编码基因、22 个转移 RNA 基因、2 个核糖体 RNA 基因和 2 个非编码区（NCR），这与其他吸虫的一致。蛋白编码和核糖体 RNA 基因在鹿平腹吸虫线粒体基因组中的排列与其他吸虫相同，除了一组血吸虫表现出衍生的排列。一些转移 RNA 基因的位置不同。基于 12 个蛋白编码基因的核苷酸序列和氨基酸序列的贝叶斯系统发育分析，将鹿平腹吸虫置于片形科内，但该科内的关系与以前的研究预期并不完全一致。鹿平腹吸虫的完整 mtDNA 序列为吸虫的诊断、生态学和进化研究提供了重要的遗传标记。
