Hinshelwood M M, Kamel F, Dierschke D J, Hauser E R
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
Domest Anim Endocrinol. 1991 Jan;8(1):37-54. doi: 10.1016/0739-7240(91)90038-l.
In order to determine the role of follicle-stimulating hormone (FSH) on the resumption of ovarian function in cows early postpartum (PP), bovine follicular fluid (FF) was used to selectively suppress concentrations of FSH. Calves were removed from all cows within 24 hr of birth. Follicular fluid that was treated with charcoal to remove steroids (15 ml; n = 14) or serum (S) from an ovariectomized cow (15 ml, n = 14) was injected i.m. twice daily from days 1 to 10 PP. Blood samples were collected before each injection and frequent samples (every 15 min for 6 hr) taken on days 5 and 10 PP. Eight cows from each group (FF and S) were slaughtered on the morning of day 11 PP and pituitaries and ovaries collected. The remaining cows (n = 6) were observed for estrus. Treatment with FF delayed follicular growth (P less than 0.01), as evidenced by the largest follicle per cow observed at time of slaughter (3.6 +/- 0.42 vs 11.5 +/- 1.77 mm dia; FF vs S). The intervals from parturition to first estrus (P less than 0.11) and to first progesterone rise (25.3 +/- 1.97 vs 18.0 +/- 3.62 d; P less than 0.06) tended to be delayed by treatment with FF vs S. Many of the cows treated with S ovulated by day 10 PP, they were divided retrospectively into those that had ovulated by (n = 9) or after (n = 5) day 10 PP for analysis. Cows treated with FF had lower (P less than 0.05) and less variable (P less than 0.01) serum FSH concentrations while levels of luteinizing hormone (LH) tended (P less than 0.08) to be greater on days 5 and 10 PP. Follicular fluid decreased levels of FSH (P less than 0.001), but not LH (P less than 0.15), in the samples obtained twice daily compared to S-treated cows that did not ovulate by day 10 PP. Anterior pituitaries were dissociated, and cells from each cow were cultured in order to ascertain whether treatment with FF in vivo would affect gonadotropin secretion in vitro. Estradiol-17 beta (E) was incubated with pituitary cells to determine the effect of E on gonadotropin secretion from cells of PP cows, and to ascertain whether treatment with FF in vivo and with E in vitro would interact to affect secretion of FSH and LH in culture. After 2 d of incubation, cells were treated with 10(-9) M E or vehicle (1% ethanol).(ABSTRACT TRUNCATED AT 400 WORDS)
为了确定促卵泡激素（FSH）在奶牛产后早期（PP）卵巢功能恢复中的作用，使用牛卵泡液（FF）选择性抑制FSH浓度。犊牛在出生后24小时内从所有奶牛身边移走。用活性炭处理以去除类固醇的卵泡液（15毫升；n = 14）或来自去卵巢奶牛的血清（S）（15毫升，n = 14）在产后第1至10天每天肌肉注射两次。在每次注射前采集血样，并在产后第5天和第10天频繁采样（每15分钟一次，共6小时）。每组（FF和S）中的8头奶牛在产后第11天上午屠宰，并采集垂体和卵巢。观察其余奶牛（n = 6）的发情情况。FF处理延迟了卵泡生长（P < 0.01），屠宰时每头奶牛观察到的最大卵泡直径可证明这一点（3.6 ± 0.42对11.5 ± 1.77毫米；FF对S）。与S处理组相比，FF处理组从分娩到首次发情（P < 0.11）和到首次孕酮升高（25.3 ± 1.97对18.0 ± 3.62天；P < 0.06）的间隔往往延迟。许多S处理的奶牛在产后第10天排卵，回顾性地将它们分为在产后第10天之前（n = 9）或之后（n = 5）排卵的奶牛进行分析。FF处理的奶牛血清FSH浓度较低（P < 0.05）且变化较小（P < 0.01），而在产后第5天和第10天促黄体生成素（LH）水平往往较高（P < 0.08）。与在产后第10天未排卵的S处理奶牛相比，每天两次采集的样本中，卵泡液降低了FSH水平（P < 0.001），但未降低LH水平（P < 0.15）。将垂体前叶解离，培养每头奶牛的细胞，以确定体内FF处理是否会影响体外促性腺激素的分泌。将雌二醇-17β（E）与垂体细胞一起孵育，以确定E对产后奶牛细胞促性腺激素分泌的影响，并确定体内FF处理和体外E处理是否会相互作用影响培养物中FSH和LH的分泌。孵育2天后，用10⁻⁹ M E或溶剂（1%乙醇）处理细胞。（摘要截断于400字）
