Gupta S M, Bala M, Muralidhar S, Ray K
Training and Research Centre, Vardhnman Mahavir Medical College and Safdarjang Hospital, New Delhi, India.
Eur J Clin Microbiol Infect Dis. 2009 May;28(5):461-8. doi: 10.1007/s10096-008-0651-y. Epub 2008 Nov 1.
Test results of syphilis serology are critical due to inter- and intralaboratory variability. The accuracy of results is crucial in the control of the disease. This study was aimed to compare results of laboratories and performance of methods for syphilis serology. Results of Veneral Disease Reference Laboratory (VDRL) or rapid plasma reagin (RPR) tests of 25 microbiology laboratories were compared in six proficiency testing sentinel surveys. The performance of laboratories was compared with the results of a hypothetical average laboratory. Of the 141 participants, 104 (73.8%) responded. The inter-laboratory variability was exhibited by 62.2% of samples tested by VDRL and 58.7% by RPR test. Intralaboratory variability was observed in 46.7% samples. There was an insignificant improvement in proficiency testing (PT) performance over the years with a significant improvement in 2007. The common errors involved departure from testing protocols. Syphilis serology proficiency testing is needed in India for reliability in test results.
由于实验室间和实验室内的变异性，梅毒血清学检测结果至关重要。结果的准确性对于疾病控制至关重要。本研究旨在比较各实验室梅毒血清学检测结果及方法性能。在六次能力验证定点调查中，对25个微生物实验室的性病研究实验室（VDRL）或快速血浆反应素（RPR）检测结果进行了比较。将各实验室的表现与一个假设的平均实验室结果进行比较。141名参与者中，104名（73.8%）做出了回应。VDRL检测的样本中有62.2%表现出实验室间变异性，RPR检测的样本中有58.7%表现出实验室间变异性。在46.7%的样本中观察到实验室内变异性。多年来能力验证（PT）表现有不显著的改善，2007年有显著改善。常见错误包括偏离检测方案。印度需要进行梅毒血清学能力验证以确保检测结果的可靠性。
