de Jong J G, Hasselman J J, van Landeghem A A, Vader H L, Wevers R A
Institute of Neurology, University of Nijmegen, St. Radboud Hospital, The Netherlands.
Clin Chem. 1991 Apr;37(4):572-5.
To check the reliability of the Ames MPS paper spot test, which is based on the Azure A dye, we sent a series of urine samples to three laboratories where the spot test is part of the metabolic screening for mucopolysaccharidoses. In these laboratories false-negative results ranged between 19% and 35% and false-positive results ranged between 12% and 29% of all samples submitted. In contrast, the quantitative dimethylmethylene blue test (Clin Chem 1989;35:1472-7) detected an increased glycosaminoglycan content in all urine samples from mucopolysaccharidosis patients and gave no false-positive results. In the latter procedure, glycosaminoglycan content is expressed per millimole of creatinine, and age-dependent reference values are used. We conclude that the Ames spot test and other spot tests are unreliable as a screening procedure for mucopolysaccharidoses and should not be used to screen for these diseases.
为检验基于天青A染料的艾姆斯MPS纸片斑点试验的可靠性，我们将一系列尿液样本送至三个实验室，在这些实验室中，斑点试验是黏多糖贮积症代谢筛查的一部分。在这些实验室中，所有送检样本的假阴性结果在19%至35%之间，假阳性结果在12%至29%之间。相比之下，定量二甲基亚甲蓝试验（《临床化学》1989年；35:1472 - 1477）在黏多糖贮积症患者的所有尿液样本中均检测到糖胺聚糖含量增加，且未出现假阳性结果。在后一种检测方法中，糖胺聚糖含量以每毫摩尔肌酐表示，并使用年龄相关的参考值。我们得出结论，艾姆斯斑点试验和其他斑点试验作为黏多糖贮积症的筛查方法不可靠，不应被用于这些疾病的筛查。
