Wang Jue, Xu Ruixia, Lin Fuqiang, Zhang Shiju, Zhang Gang, Hu Shengshou, Zheng Zhe
Research Center for Cardiovascular Regenerative Medicine, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
Cardiology. 2009;113(2):81-8. doi: 10.1159/000172616. Epub 2008 Nov 19.
MicroRNAs (miRNAs) may serve as potential diagnostic biomarkers and therapeutic targets in cardiovascular research. However, the association between miRNAs and heart remodeling/reverse remodeling has not been evaluated.
Lewis rats were divided into three groups: control animals, animals subjected to abdominal aortic constriction (AAC) and those with heterotopic transplantation of abdominal aortic constriction (AAC-HT), respectively. The cardiomyocyte cross-sectional area and changes in the heart cavity were determined. miRNA microarray was used to search for changes in miRNAs during hypertrophy and in the unloading heart, which was also verified using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).
The AAC-HT group exhibited a 54% decrease in the cardiomyocyte cross-sectional area compared to the AAC group. In the two test groups, miRNA microarrays revealed changes in 293 miRNAs, among which 40 miRNAs changed >2-fold. Some major changes were also confirmed using qRT-PCR primers. The results indicated that changes in miR-23a and miR-29a were most significant, thus suggesting that these miRNAs may play important roles in heart remodeling and reverse remodeling.
The changes observed in miRNA expression during hypertrophy and reverse remodeling may indicate possibly meaningful targets for regulating the remodeling or reverse remodeling of the heart.
微小RNA（miRNA）可能作为心血管研究中潜在的诊断生物标志物和治疗靶点。然而，miRNA与心脏重塑/逆向重塑之间的关联尚未得到评估。
将Lewis大鼠分为三组：对照组动物、接受腹主动脉缩窄（AAC）的动物以及接受腹主动脉缩窄异位移植（AAC-HT）的动物。测定心肌细胞横截面积和心腔变化。使用miRNA微阵列来寻找肥大和卸载心脏过程中miRNA的变化，并通过实时定量逆转录聚合酶链反应（qRT-PCR）进行验证。
与AAC组相比，AAC-HT组的心肌细胞横截面积减少了54%。在两个测试组中，miRNA微阵列显示293种miRNA发生了变化，其中40种miRNA变化超过2倍。使用qRT-PCR引物也证实了一些主要变化。结果表明，miR-23a和miR-29a的变化最为显著，因此表明这些miRNA可能在心脏重塑和逆向重塑中发挥重要作用。
在肥大和逆向重塑过程中观察到的miRNA表达变化可能表明是调节心脏重塑或逆向重塑的潜在有意义靶点。
