以煤油作为唯一碳源进行固氮生长的黑褐固氮菌的包囊形成。

Encystment of Azotobacter nigricans grown diazotrophically on kerosene as sole carbon source.

作者信息

García-Esquivel Gabriela, Calva-Calva Graciano, Ferrera-Cerrato Ronald, Fernández-Linares Luis Carlos, Vázquez Refugio Rodríguez, Esparza-García Fernando José

机构信息

Biotechnology and Bioengineering Department, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, C. P. 07360 Mexico D. F., Mexico.

出版信息

Arch Microbiol. 2009 Mar;191(3):275-81. doi: 10.1007/s00203-008-0444-9. Epub 2008 Nov 19.

DOI:10.1007/s00203-008-0444-9

PMID:19018516

Abstract

Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was higher and the poly-beta-hydroxybutyrate granules were not present to a significant extent, peripheral bodies were abundant. After 8 days of culture on kerosene, the presence of cysts with intracellular bunches of poly-beta-hydroxybutyrate granules was observed. Germination of cysts bears germinating multicelled yet unbroken capsule cysts with up to three cells inside. This is the first report of encystment induction of Azotobacter species grown on kerosene.

摘要

通过在煤油上进行固氮培养诱导黑褐固氮菌形成孢囊。与在蔗糖上培养的菌相比，煤油对其生长和固氮酶活性有影响。营养细胞的电子显微镜观察表明，当固氮酶活性较高且聚-β-羟基丁酸酯颗粒不存在显著程度时，外周体丰富。在煤油上培养8天后，观察到含有细胞内聚-β-羟基丁酸酯颗粒束的孢囊。孢囊萌发产生萌发的多细胞但未破裂的胶囊状孢囊，内部有多达三个细胞。这是关于在煤油上生长的固氮菌属诱导形成孢囊的首次报道。