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[利用不同碳源底物对褐球固氮菌进行包囊化]

[Encystment using different carbon substrates in Azotobacter chroococcum].

作者信息

Marengo G W

机构信息

Cátedra de Microbiología, Facultad de Agronomía, Universidad Nacional de Rosario, Argentina.

出版信息

Rev Argent Microbiol. 1983;15(3):143-6.

PMID:6400765
Abstract

Carbon nutrition has a fundamental role in the encystment of bacteria of the genus Azotobacter. The effect of liquid media with various organic carbon substrates on the encystment of 2 strains of Azotobacter chroococcum was studied. Both strains had been previously cultured in a glucose and mannitol liquid medium. Strain 2087 showed the greatest degree of encystment (78%) with isopropanol and a very low percentage of cyst formation in the glucose and mannitol medium. In strain 1847 an important percentage of cyst formation (33%) was obtained in the glucose and mannitol medium and no cysts appeared with isopropanol. N-butanol and N-propanol induced in both strains relatively reduced percentages of encystment. The differential response found in the glucose and mannitol medium and the isopropanol media with strains 2087 and 1847 of A. chroococcum, has a degree of similarity in the different intensity of encystment shown by diverse strains of A. vinelandii with certain carbon substrates.

摘要

碳营养在固氮菌属细菌的孢囊形成过程中起着重要作用。研究了含有各种有机碳底物的液体培养基对两株褐球固氮菌孢囊形成的影响。这两株菌之前都在葡萄糖和甘露醇液体培养基中培养过。2087菌株在异丙醇培养基中表现出最高的孢囊形成率(78%),而在葡萄糖和甘露醇培养基中孢囊形成率非常低。1847菌株在葡萄糖和甘露醇培养基中获得了较高的孢囊形成率(33%),在异丙醇培养基中未出现孢囊。正丁醇和正丙醇在两株菌中均导致相对较低的孢囊形成率。在褐球固氮菌2087和1847菌株中,葡萄糖和甘露醇培养基以及异丙醇培养基之间的差异反应,与不同菌株的棕色固氮菌在某些碳底物上表现出的不同程度的孢囊形成强度具有一定的相似性。

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