Hong Y F, Kang J G, Lee H Y, Uhm H S, Moon E, Park Y H
Department of Molecular Science and Technology, Ajou University, Youngtong-Gu, Suwon, Korea.
Lett Appl Microbiol. 2009 Jan;48(1):33-7. doi: 10.1111/j.1472-765X.2008.02480.x. Epub 2008 Nov 19.
Escherichia coli and Bacillus subtilis spores were treated with an atmospheric plasma mixture created by the ionization of helium and oxygen to investigate the inactivation efficiency of a low-temperature plasma below 70 degrees C.
An electrical discharge plasma was produced at a radio frequency (RF) of 13.56 MHz, connected to a perforated circular electrode with a discharge spacing of 1-15 mm. The discharge gas was helium with 0-2% oxygen. For the plasma treatment, a dried E. coli cell or B. subtilis endospore suspension on a cover-glass was exposed to oxygen downstream of the plasma from holes in an RF-powered electrode. The sterilization effect of the RF plasma was highest with 0.2% oxygen, corresponding to the maximum production of oxygen radicals.
Oxygen radicals generated by RF plasma are effective for the destruction of bacterial cells and endospores.
Low-temperature atmospheric plasma can be used for the disinfection of diverse objects, especially for the inactivation of bacterial endospores.
用由氦气和氧气电离产生的常压等离子体混合物处理大肠杆菌和枯草芽孢杆菌孢子，以研究70摄氏度以下低温等离子体的灭活效率。
在13.56兆赫兹的射频下产生放电等离子体，连接到一个放电间距为1 - 15毫米的多孔圆形电极。放电气体是含有0 - 2%氧气的氦气。对于等离子体处理，将盖玻片上的干燥大肠杆菌细胞或枯草芽孢杆菌芽孢悬液从射频供电电极的孔暴露于等离子体下游的氧气中。射频等离子体在氧气含量为0.2%时杀菌效果最佳，这与氧自由基的最大产生量相对应。
射频等离子体产生的氧自由基对破坏细菌细胞和芽孢有效。
低温常压等离子体可用于多种物体的消毒，尤其用于灭活细菌芽孢。
