College of Food Sciences & Engineering, Hainan University, 58 People Road, Haikou 570228, China.
Int J Mol Sci. 2024 Jul 18;25(14):7875. doi: 10.3390/ijms25147875.
Direct barrier discharge (DBD) plasma is a potential antibacterial strategy for controlling () in the food industry. The aim of this study was to investigate the inhibitory effect and mechanism of action of DBD plasma on . The result of the antibacterial effect curve shows that DBD plasma has a good inactivation effect on . The DBD plasma treatment severely disrupted the cell membrane structure and resulted in the leakage of intracellular components. In addition, flow cytometry was used to observe intracellular reactive oxygen species (ROS) levels and mitochondrial membrane potential, and it was found that, after plasma treatment, intracellular ROS accumulation and mitochondrial damage were accompanied by a decrease in antioxidant enzyme activity. The results of free fatty acid metabolism indicate that the saturated fatty acid content increased and unsaturated fatty acid content decreased. Overall, the DBD plasma treatment led to the oxidation of unsaturated fatty acids, which altered the cell membrane fatty acid content, thereby inducing cell membrane damage. Meanwhile, DBD plasma-induced ROS penetrated the cell membrane and accumulated intracellularly, leading to the collapse of the antioxidant system and ultimately causing cell death. This study reveals the bactericidal effect and mechanism of the DBD treatment on , which provides a possible strategy for the control of .
直接屏障放电 (DBD) 等离子体是控制食品工业中 () 的潜在抗菌策略。本研究旨在研究 DBD 等离子体对 () 的抑制作用及其作用机制。抑菌效果曲线的结果表明，DBD 等离子体对具有良好的失活动力。DBD 等离子体处理严重破坏了细胞膜结构，导致细胞内成分泄漏。此外，还通过流式细胞术观察了细胞内活性氧 (ROS) 水平和线粒体膜电位，发现等离子体处理后，伴随着抗氧化酶活性的降低，细胞内 ROS 积累和线粒体损伤。游离脂肪酸代谢的结果表明，饱和脂肪酸含量增加，不饱和脂肪酸含量减少。总的来说，DBD 等离子体处理导致不饱和脂肪酸的氧化，改变了细胞膜脂肪酸含量，从而诱导细胞膜损伤。同时，DBD 等离子体诱导的 ROS 穿透细胞膜并在细胞内积累，导致抗氧化系统崩溃，最终导致细胞死亡。本研究揭示了 DBD 处理对 () 的杀菌效果及其机制，为控制 () 提供了一种可能的策略。
