Seo S, King J M, Prinyawiwatkul W, Janes M
Dept. of Food Science, 111 Food Science Bldg., Louisiana State Univ. Agricultural Center, Baton Rouge, LA 70803, USA.
J Food Sci. 2008 Oct;73(8):M400-4. doi: 10.1111/j.1750-3841.2008.00922.x.
Antimicrobial activities of chitosan samples with different molecular weights (1333, 432, 201, 131, and 104 kDa) prepared by ozone treatment were examined against 2 Gram-positive bacteria (Listeria monocytogenes and Staphylococcus aureus) and 2 Gram-negative bacteria (Escherichia coli and Pseudomonas fluorescen) to investigate the effect of chitosan's molecular weight and concentration on the inhibition of bacterial growth. Antimicrobial activity of chitosan varied depending on the molecular weight, concentration of chitosan, and type of microorganism. Generally, the effectiveness of the chitosans significantly increased with increasing chitosan concentration, regardless of molecular size and types of bacteria. Chitosan with molecular weights ranging from 104 to 201 kDa showed relatively greater antimicrobial activity against L. monocytogenes, S. aureus, and P. fluorescen; whereas for E. coli, intermediate molecular weight chitosan was more effective in growth inhibition than lower or higher molecular weight chitosan particularly at 0.1% concentration.
研究了经臭氧处理制备的不同分子量（1333、432、201、131和104 kDa）壳聚糖样品对2种革兰氏阳性菌（单核细胞增生李斯特菌和金黄色葡萄球菌）和2种革兰氏阴性菌（大肠杆菌和荧光假单胞菌）的抗菌活性，以考察壳聚糖分子量和浓度对细菌生长抑制的影响。壳聚糖的抗菌活性因分子量、壳聚糖浓度和微生物类型而异。一般来说，无论分子量大小和细菌类型如何，壳聚糖的有效性均随壳聚糖浓度的增加而显著提高。分子量在104至201 kDa之间的壳聚糖对单核细胞增生李斯特菌、金黄色葡萄球菌和荧光假单胞菌表现出相对较强的抗菌活性；而对于大肠杆菌，中等分子量的壳聚糖在抑制生长方面比低分子量或高分子量的壳聚糖更有效，尤其是在0.1%浓度时。
