Konstantinov Konstantin N, Sitdikov Ravil A, Lopez Gabriel P, Atanassov Plamen, Rubin Robert L
Dept. of Internal Medicine, University of New Mexico Health Sciences Center, USA.
Biosens Bioelectron. 2009 Mar 15;24(7):1949-54. doi: 10.1016/j.bios.2008.09.032. Epub 2008 Oct 17.
The current system employed electrochemical sensor technology for the measurement of autoantibodies in human sera. Anti-chromatin antibodies are an early and sensitive indicator of systemic lupus erythematosus (SLE) and are typically quantified using enzyme-linked immunosorbent assays (ELISA). Electrochemical detection compared favorably with ELISA using a sample of 30 SLE sera (r=0.9), and non-specific binding by normal serum immunoglobulin was undetectable. The electrochemical sensor assay required <20min processing time and utilized a hand-held apparatus with a disposable electrode. These results demonstrate the applicability of this technology to the rapid measurement of a clinically relevant analyte with an apparatus of potential simplicity and low cost.
当前系统采用电化学传感器技术来测量人血清中的自身抗体。抗染色质抗体是系统性红斑狼疮（SLE）的早期敏感指标，通常使用酶联免疫吸附测定（ELISA）进行定量。使用30份SLE血清样本进行检测时，电化学检测与ELISA相比效果良好（r = 0.9），且未检测到正常血清免疫球蛋白的非特异性结合。电化学传感器检测所需的处理时间不到20分钟，并且使用了带有一次性电极的手持设备。这些结果证明了该技术适用于使用可能简单且低成本的设备快速测量临床相关分析物。
