Chiba Shota, Jiang Di, Satoh Noriyuki, Smith William C
Department of Molecular, Cell and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA.
Development. 2009 Jan;136(1):35-9. doi: 10.1242/dev.030981. Epub 2008 Nov 19.
We report the isolation of a recessive ENU-induced short-tailed mutant in the ascidian Ciona intestinalis that is the product of a premature stop in the brachyury gene. Notochord differentiation and morphogenesis are severely disrupted in the mutant line. At the larval stage, variable degrees of ectopic endoderm staining were observed in the homozygous mutants, indicating that loss of brachyury results in stochastic fate transformation. In post-metamorphosis mutants, a uniform defect in tail resorption was observed, together with variable defects in digestive tract development. Some cells misdirected from the notochord lineage were found to be incorporated into definitive endodermal structures, such as stomach and intestine.
我们报告了在海鞘Ciona intestinalis中分离出一种隐性ENU诱导的短尾突变体，它是短尾基因中过早出现终止密码子的产物。在突变系中，脊索分化和形态发生受到严重破坏。在幼虫阶段，在纯合突变体中观察到不同程度的异位内胚层染色，这表明短尾基因的缺失导致了随机的命运转变。在变态后的突变体中，观察到尾部吸收存在一致的缺陷，同时消化道发育也存在不同程度的缺陷。一些从脊索谱系中错误定向的细胞被发现整合到了确定的内胚层结构中，如胃和肠。
