Pauli U, Zanoni R, Hertig C, Peterhans E
Institut für Veterinär-Virologie, Universität Bern.
Schweiz Arch Tierheilkd. 1991;133(1):35-42.
In the last years molecular biology has gained more and more influence in veterinary medicine, e.g. in animal breeding. In addition, methods of molecular biology may open new areas in the diagnosis of viral diseases, because they are fast and very sensitive. The following article displays an overview of molecular biological techniques that are currently in use for virus detection. In addition, the limits of these methods are discussed. In particular, the polymerase chain reaction (PCR) is described in some detail. Since this method has a considerable impact on the progress in molecular biology, it may as well be of importance for the detection of viruses. The potential of PCR in virus detection is illustrated using sheep and goat lentivirus and Bovine Viral Diarrhea/Mucosal Disease virus.
近年来,分子生物学在兽医学领域的影响力越来越大,例如在动物育种方面。此外,分子生物学方法可能会为病毒性疾病的诊断开辟新领域,因为它们快速且极为灵敏。以下文章概述了目前用于病毒检测的分子生物学技术。此外,还讨论了这些方法的局限性。特别详细描述了聚合酶链反应(PCR)。由于该方法对分子生物学的进展有重大影响,它对病毒检测可能也很重要。通过绵羊和山羊慢病毒以及牛病毒性腹泻/黏膜病病毒举例说明了PCR在病毒检测中的潜力。