Pop Cristian V L, Neamtu Silvia
National Institute for Research and Development of Isotopic and Molecular Technologies, Molecular and Biomolecular Physics Department, 71-103 Donath Street, 400293 Cluj-Napoca, Romania.
J Biomed Opt. 2008 Jul-Aug;13(4):041308. doi: 10.1117/1.2956658.
Red blood cells (RBCs) in the presence of plasma proteins or other macromolecules have a tendency to form aggregates. Light-scattering technique was used to investigate the RBC aggregation process. A highly diluted suspension of RBCs was illuminated with a 632.8-nm HeNe laser. Angular-resolved measurements of light intensity scattered by an RBC suspension from a 200-microm thick optical glass cuvette during 10 min of their aggregation process were performed at 1 to 4 off-axis deg with a very high angular resolution, at hematocrits in the range of 3.5 x 10(-2) to 10(-1). The angular spreading of forward-scattered light at small angles during the RBC aggregation process was described in terms of a new, effective phase function model that has been used for fitting the experimental data. The aggregated RBCs' optical properties, such as effective scattering anisotropy and scattering cross section, were determined. The results were compared with prediction of Mie theory for equivolumetric spherical particles. The time dependence of the aggregates mean radius and of the mean number of cells per aggregate was also calculated. Last, the potential of the proposed technique (forward-scattering light technique) as a new quantitative investigation of cellular aggregation process was estimated.
在存在血浆蛋白或其他大分子的情况下，红细胞（RBCs）有形成聚集体的倾向。采用光散射技术研究红细胞聚集过程。用632.8纳米的氦氖激光照射高度稀释的红细胞悬液。在红细胞聚集过程的10分钟内，对来自200微米厚光学玻璃比色皿的红细胞悬液散射的光强度进行角分辨测量，测量角度为离轴1至4度，角分辨率非常高，血细胞比容范围为3.5×10⁻²至10⁻¹。用一个新的有效相位函数模型描述了红细胞聚集过程中小角度前向散射光的角展宽，该模型已用于拟合实验数据。测定了聚集红细胞的光学性质，如有效散射各向异性和散射截面。将结果与等体积球形颗粒的米氏理论预测进行了比较。还计算了聚集体平均半径和每个聚集体平均细胞数的时间依赖性。最后，评估了所提出的技术（前向散射光技术）作为细胞聚集过程新的定量研究方法的潜力。
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