对悬浮液中红细胞在小角度散射光的高分辨率角分辨测量。

High-resolution angle-resolved measurements of light scattered at small angles by red blood cells in suspension.

作者信息

Turcu Ioan, Pop Cristian V L, Neamtu Silvia

机构信息

National Institute for Research and Development of Isotopic and Molecular Technologies, P.O. Box 700, RO-400293 Cluj-Napoca, Romania.

出版信息

Appl Opt. 2006 Mar 20;45(9):1964-71. doi: 10.1364/ao.45.001964.

DOI:10.1364/ao.45.001964

PMID:16579566

Abstract

Red blood cells (RBCs) scatter light mainly in the forward direction, where the scattering phase function has a narrow peak. We performed an experimental investigation into the angular distribution of light scattered by blood in the small-angle domain. A highly diluted suspension of RBCs (hematocrits in the range 5 x 10(-5)-10(-2)) was illuminated with a He-Ne laser with 633 nm wavelength. We focused our research on two main topics: the scattering efficiency of the RBCs given by the mean scattering cross section and the scattering anisotropy obtained from the angular distribution of the scattered photons. The collimated beam transmission and the angular distribution of scattered light were measured and compared with the predictions of the effective phase function model. The RBCs' mean scattering cross section and scattering anisotropy were obtained by fitting of the experimental data.

摘要

红细胞（RBCs）主要在向前的方向散射光，在该方向散射相位函数有一个窄峰。我们对小角度范围内血液散射光的角度分布进行了实验研究。用波长为633nm的氦氖激光照射高度稀释的红细胞悬浮液（血细胞比容范围为5×10⁻⁵ - 10⁻²）。我们将研究重点集中在两个主要方面：由平均散射截面给出的红细胞散射效率以及从散射光子的角度分布获得的散射各向异性。测量了准直光束透射率和散射光的角度分布，并与有效相位函数模型的预测结果进行了比较。通过对实验数据进行拟合得到了红细胞的平均散射截面和散射各向异性。