Salyer David A, Denninghoff Kurt R, Beaudry Neil, Basavanthappa Sreenivasa, Park Robert I, Chipman Russell A
University of Arizona, Optical Sciences Center, Tucson, Arizona 85721, USA.
J Biomed Opt. 2008 Jul-Aug;13(4):044004. doi: 10.1117/1.2966953.
The diffuse fundus reflectance and the spectral transmittance of the swine sensory retina was measured in vivo using intravitreal illumination. Pars plana vitrectomy and intravitreal manipulations were performed on a female American Yorkshire domestic swine. Light from a scanning monochromator was coupled into a fiber optic intraocular illuminator inserted into the vitreous. A 1.93-mm(2) region of the illuminated fundus was imaged from an oblique illumination angle. Multispectral retinal images were acquired for four experimental conditions: the eye (1) prior to vitrectomy, (2) after vitrectomy, (3) after insertion of a Spectralon disk super-retinally, and (4) after subretinal insertion of the disk. The absorption of melanin and hemoglobin in the red wavelengths was used to convert relative spectral reflectance to absolute reflectance. The flux scattered from the super-retinal Spectralon was used to correct for scattering in the globe. The transmittance of the sensory retina was measured in vivo using the scatter corrected subretinal Spectralon disk reflectance. The hemoglobin and melanin components of the spectrum due to scattered light were removed from the retinal transmission spectrum. The in vivo spectral transmittance of the sensory retina in this swine was essentially flat across the visible spectrum, with an average transmittance >90%.
利用玻璃体腔内照明在体测量猪感觉视网膜的漫反射眼底和光谱透射率。对一头雌性美国约克夏家猪进行了玻璃体平坦部玻璃体切除术和玻璃体腔内操作。来自扫描单色仪的光耦合到插入玻璃体的光纤眼内照明器中。从倾斜照明角度对被照明眼底的一个1.93平方毫米的区域进行成像。针对四种实验条件采集多光谱视网膜图像：眼睛（1）在玻璃体切除术前，（2）在玻璃体切除术后，（3）在视网膜上插入Spectralon盘后，以及（4）在视网膜下插入盘后。利用红色波长中黑色素和血红蛋白的吸收将相对光谱反射率转换为绝对反射率。从视网膜上的Spectralon散射的通量用于校正眼球内的散射。利用经散射校正的视网膜下Spectralon盘反射率在体测量感觉视网膜的透射率。从视网膜透射光谱中去除由于散射光导致的光谱中的血红蛋白和黑色素成分。该猪感觉视网膜的体内光谱透射率在可见光谱范围内基本平坦，平均透射率>90%。
