牛蛙（美国牛蛙）血清极低密度脂蛋白和高密度脂蛋白的化学特性

Chemical characterization of the serum very low density and high density lipoproteins from bullfrog, Rana catesbeiana.

作者信息

Suzuki N, Deguchi K, Ueta N, Nagano H, Shukuya R

出版信息

J Biochem. 1976 Dec;80(6):1241-6. doi: 10.1093/oxfordjournals.jbchem.a131395.

DOI:10.1093/oxfordjournals.jbchem.a131395

PMID:190215

Abstract

The chemical properties of very low density and high density lipoproteins of adult bullfrog serum were determined. This serum contained extremely low levels of both very low density lipoprotein (10-30 mg/100 ml) and high density lipoprotein (5-10 mg/100 ml). The constituents of very low density lipoprotein, on a weight percentage basis, were found to be 48.1% triglyceride, 17.3% cholesterol ester, 8.8% cholesterol, 11.6% phospholipid, and 12% protein. These constituents were also present in high density lipoprotein with weight percentage values of 3.7%, 19.3%, 11.9%, 25.2%, and 36.8%, respectively. The fatty acid compositions of the triglycerides, cholesterol esters, and phosphatidylcholine were quite similar in the very low density lipoprotein and high density lipoprotein. However, shingomyelin fatty acid composition was appreciably different in the two lipoproteins. Disc gel electrophoresis in sodium dodecyl sulfate-polyacrylamide gels produced patterns with one major (approximate molecular weight, 7,000) and several minor bands for the apoprotein of very low density lipoprotein and one major (approximate molecular weight, 28,000) and several minor bands for that of high density lipoprotein.

摘要

测定了成年牛蛙血清中极低密度脂蛋白和高密度脂蛋白的化学性质。该血清中极低密度脂蛋白（10 - 30毫克/100毫升）和高密度脂蛋白（5 - 10毫克/100毫升）的含量都极低。以重量百分比计，极低密度脂蛋白的成分如下：甘油三酯48.1%、胆固醇酯17.3%、胆固醇8.8%、磷脂11.6%和蛋白质12%。这些成分在高密度脂蛋白中也有，其重量百分比分别为3.7%、19.3%、11.9%、25.2%和36.8%。极低密度脂蛋白和高密度脂蛋白中甘油三酯、胆固醇酯和磷脂酰胆碱的脂肪酸组成相当相似。然而，两种脂蛋白中鞘磷脂的脂肪酸组成明显不同。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶中进行圆盘凝胶电泳，极低密度脂蛋白载脂蛋白产生了一条主要带（近似分子量7000）和几条次要带，高密度脂蛋白载脂蛋白产生了一条主要带（近似分子量28000）和几条次要带。