Goldberg Martin W, Fiserova Jindriska, Huttenlauch Irm, Stick Reimer
School of Biological and Biomedical Sciences, Durham University, Sciences Laboratories, South Road, Durham, UK.
Biochem Soc Trans. 2008 Dec;36(Pt 6):1339-43. doi: 10.1042/BST0361339.
Lamins are intermediate filament proteins that form a network lining the inner nuclear membrane. They provide mechanical strength to the nuclear envelope, but also appear to have many other functions as reflected in the array of diseases caused by lamin mutations. Unlike other intermediate filament proteins, they do not self-assemble into 10 nm filaments in vitro and their in vivo organization is uncertain. We have recently re-examined the organization of a simple B-type lamina in Xenopus oocytes [Goldberg, Huttenlauch, Hutchison and Stick (2008) J. Cell Sci. 121, 215-225] and shown that it consists of tightly packed 8-10 nm filaments with regular cross-connections, tightly opposed to the membrane. When lamin A is expressed in oocytes, it forms organized bundles on top of the B lamina. This has led to a new model for lamina organization which is discussed in the present paper.
核纤层蛋白是中间丝蛋白，它们在内核膜内衬形成一个网络。它们为核膜提供机械强度，但正如由核纤层蛋白突变引起的一系列疾病所反映的那样，它们似乎还有许多其他功能。与其他中间丝蛋白不同，它们在体外不会自组装成10纳米的细丝，其体内组织情况尚不确定。我们最近重新研究了非洲爪蟾卵母细胞中简单B型核纤层的组织情况[戈德堡、胡滕劳赫、哈钦森和施蒂克（2008年）《细胞科学杂志》121卷，215 - 225页]，结果表明它由紧密排列的8 - 10纳米细丝组成，这些细丝有规则的交叉连接，与膜紧密相对。当在卵母细胞中表达核纤层蛋白A时，它会在B型核纤层之上形成有组织的束状物。这导致了一种新的核纤层组织模型，本文将对此进行讨论。
