Pilavtepe-Celik M, Balaban M O, Alpas H, Yousef A E
Vocational School of Ihsaniye, Kocaeli University, 41040, Izmit, Kocaeli, Turkey.
J Food Sci. 2008 Nov;73(9):M423-9. doi: 10.1111/j.1750-3841.2008.00947.x.
Scanning electron microscopy (SEM) images of Staphylococcus aureus 485 and Escherichia coli O157:H7 933 were taken after pressure treatments at 200 to 400 MPa. Software developed for this purpose was used to analyze SEM images and to calculate the change in view area and volume of cells. Significant increase in average cell view area and volume for S. aureus 485 was observed in response to pressure treatment at 400 MPa. Cell view area for E. coli O157:H7 933 significantly increased at 325 MPa, the maximum pressure treatment tested against this pathogen. In contrast to S. aureus, cells of E. coli O157:H7 exhibited significant increase in average view area and volume at 200 MPa. The pressure-induced increase in these parameters may be attributed to modifications in membrane properties, for example, denaturation of membrane-bound proteins and pressure-induced phase transition of membrane lipid bilayer.
在200至400兆帕的压力处理后，拍摄了金黄色葡萄球菌485株和大肠杆菌O157:H7 933株的扫描电子显微镜（SEM）图像。为此开发的软件用于分析SEM图像，并计算细胞视野面积和体积的变化。在400兆帕的压力处理下，观察到金黄色葡萄球菌485株的平均细胞视野面积和体积显著增加。大肠杆菌O157:H7 933株的细胞视野面积在325兆帕时显著增加，这是针对该病原体测试的最大压力处理。与金黄色葡萄球菌不同，大肠杆菌O157:H7的细胞在200兆帕时平均视野面积和体积显著增加。这些参数的压力诱导增加可能归因于膜性质的改变，例如膜结合蛋白的变性和膜脂双层的压力诱导相变。
