[阳离子脂质体包裹碱性成纤维细胞生长因子作为佐剂：技术参数的优化]

[BFGF entraped in cationic liposomes as adjuvant: optimization of technical parameters].

作者信息

Zhong Zhen-hua, Mao Shuang-lin, Yang Li, Yang Guang-li, Hou Wen-li, Chen Xiang, Chen Li-juan

机构信息

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Sep;39(5):839-41, 846.

PMID:19024328

Abstract

OBJECTIVE

To test the immune efficiency of bFGF entraped in cationic liposomes as adjuvant in vivo.

METHODS

The technical parameters on encapsulation were tested in each step to gain high encapsulation efficiencies, which included lipid composition, weight ratio of protein and lipids, liposome extrusion, and different conditions of freeze-thawing. The bFGF in cationic liposome, Freund's adjuvant, or PBS were injected (four times) to the four-week old Balb/c mice to test the immune responses. The serum antibody was measured by ELISA 13 days after each injection.

RESULTS

Maximal encapsulation efficiency (about 50%) was achieved through optimized technical parameters. Cationic liposome demonstrated satisfied immune efficiency as adjuvant.

CONCLUSION

Cationic liposome is a safe and effective immunological adjuvant.

摘要

目的

在体内测试包裹于阳离子脂质体中的碱性成纤维细胞生长因子（bFGF）作为佐剂的免疫效果。

方法

对包封过程中的各项技术参数进行测试以获得高包封率，这些参数包括脂质组成、蛋白质与脂质的重量比、脂质体挤压以及不同的冻融条件。将阳离子脂质体包裹的bFGF、弗氏佐剂或磷酸盐缓冲液（PBS）注射到四周龄的Balb/c小鼠体内（共注射四次）以测试免疫反应。每次注射13天后通过酶联免疫吸附测定（ELISA）法检测血清抗体。