[两种用于快速诊断轮状病毒感染的免疫层析试剂盒的评估]

[Evaluation of two immunochromatography kits for rapid diagnosis of rotavirus infections].

作者信息

Téllez C J, Montava R, Ribes Juan M, Tirado M D, Buesa J

机构信息

Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia, Hospital Clinico Universitario de Valencia, Avda. Blasco Ibáñez, 17, 46010 Valencia, España.

出版信息

Rev Argent Microbiol. 2008 Jul-Sep;40(3):167-70.

PMID:19024505

Abstract

A prospective study was conducted to evaluate two immunochromatography (ICG) commercial kits for diagnosis of rotavirus infection, VIKIA Rota-Adeno (bioMérieux) and Simple Rota-Adeno (Operon). Reverse transcriptase and polymerase chain reaction (RT-PCR) with specific primers for the VP7 gene of group A rotavirus was used as the reference method. The sensitivity and specificity of the ICG tests compared with those of the reference method were 98.4% and 84.8%, respectively, for Simple Rota-Adeno (Operon), and 100% and 24.2% for VlKIA Rota-Adeno (bioMérieux). It is remarkable the low specificity of the latter method, which yields a high number of false positive results. The predictive value of a positive result by this method was only 71.6%. Most of the detected rotavirus strains corresponded to genotype G9P[8] (65%), followed by G1P[8] (25.4%) and G2P[8] (3.2%).

摘要

进行了一项前瞻性研究，以评估两种用于诊断轮状病毒感染的免疫层析（ICG）商业试剂盒，即VIKIA Rota-Adeno（生物梅里埃公司）和Simple Rota-Adeno（奥普龙公司）。使用针对A组轮状病毒VP7基因的特异性引物进行逆转录聚合酶链反应（RT-PCR）作为参考方法。与参考方法相比，Simple Rota-Adeno（奥普龙公司）的ICG检测的敏感性和特异性分别为98.4%和84.8%，而VIKIA Rota-Adeno（生物梅里埃公司）的敏感性和特异性分别为100%和24.2%。后一种方法的特异性较低，产生大量假阳性结果，这一点很显著。该方法阳性结果的预测值仅为71.6%。检测到的大多数轮状病毒株对应于G9P[8]基因型（65%），其次是G1P[8]（25.4%）和G2P[8]（3.2%）。