Gleesen Ann Sofie, Grarup Cecilie, Dargis Rimtas, Andresen Keld, Christensen Jens Jørgen, Kemp Michael
The Laboratory for Clinical Microbiology at Statens Serum Institut, Copenhagen, Denmark.
APMIS. 2008 Sep;116(9):811-5. doi: 10.1111/j.1600-0463.2008.00793.x.
The results of partial polymerase chain reaction (PCR) amplification of the bacterial 16S rRNA gene and subsequent DNA sequencing of clinical samples from children are described. In 13 out of 62 samples, DNA from bacteria likely to be the cause of infection was identified. In the vast majority (11/13) of samples with significant pathogen culture the specimen had been negative. Antibiotics had been given in all cases except for three prior to sampling. PCR and subsequent DNA sequencing is a valuable supplementary tool for establishing the cause of bacterial infections in children when culture is negative.
本文描述了对儿童临床样本进行细菌16S rRNA基因的部分聚合酶链反应（PCR）扩增及后续DNA测序的结果。在62份样本中，有13份鉴定出可能是感染病因的细菌DNA。在绝大多数（11/13）有显著病原体培养结果的样本中，之前的标本检测为阴性。除了3例在采样前未使用抗生素外，所有病例均使用过抗生素。当培养结果为阴性时，PCR及后续DNA测序是确定儿童细菌感染病因的一项有价值的辅助工具。
