Munirah S, Samsudin O C, Chen H C, Salmah S H Sharifah, Aminuddin B S, Ruszymah B H I
Department of Physiology, Faculty of Medicine, UKM Medical Centre, Kuala Lumpur.
Med J Malaysia. 2008 Jul;63 Suppl A:35-6.
Chondrocytes were isolated from articular cartilage biopsy and were cultivated in vitro. Approximately 30 million of cultured chondrocytes per ml were incorporated with autologous plasma-derived fibrin to form three-dimensional construct. Full-thickness punch hole defects were created in lateral and medial femoral condyles. The defects were implanted either with the autologous 'chondrocytes-fibrin' construct (ACFC), autologous chondrocytes (ACI) or fibrin blank (AF). Sheep were euthanized after 12 weeks. The gross morphology of all defects treated with ACFC implantation, ACI and AF exhibited median scores which correspond to a nearly normal appearance according to the International Cartilage Repair Society (ICRS) classification. ACFC significantly enhanced cartilage repair compared to ACI and AF in accordance with the modified O'Driscoll histological scoring scale. The relative sulphated glycosaminoglycans content (%) was significantly higher (p < 0.05) in ACFC when compared to control groups; ACI vs. fibrin only vs. untreated (blank). Results showed that ACFC implantation exhibited superior cartilage-like tissue regeneration compared to ACI. If the result is applicable to the human, it possibly will improve the existing treatment approaches for cartilage restoration in orthopaedic surgery.
从关节软骨活检中分离软骨细胞并在体外培养。每毫升约3000万个培养的软骨细胞与自体血浆衍生的纤维蛋白混合，形成三维构建体。在股骨内外侧髁制造全层打孔缺损。缺损处分别植入自体“软骨细胞 - 纤维蛋白”构建体（ACFC）、自体软骨细胞（ACI）或纤维蛋白空白对照（AF）。12周后对绵羊实施安乐死。根据国际软骨修复协会（ICRS）分类，ACFC植入、ACI和AF治疗的所有缺损的大体形态表现出的中位评分对应于几乎正常的外观。根据改良的奥德里斯科尔组织学评分量表，与ACI和AF相比，ACFC显著增强了软骨修复。与对照组（ACI与仅纤维蛋白与未处理（空白）相比）相比，ACFC中的相对硫酸化糖胺聚糖含量（%）显著更高（p < 0.05）。结果表明，与ACI相比，ACFC植入表现出更好的软骨样组织再生。如果该结果适用于人类，可能会改善骨科手术中现有的软骨修复治疗方法。
