Tong C K, Seow H F, Ramasamy R
Department of Pathology, Faculty of Medicine and Health Sciences, University Putra, Malaysia.
Med J Malaysia. 2008 Jul;63 Suppl A:77-8.
The immune modulatory properties of mesenchymal stem cell (MSC) had brought a new insight in cell-based neotherapy. However, recent works of MSC are focused exclusively on bone marrow-derived MSC. We evaluated the immunogenicity of cord blood-derived MSC (CB-MSC) on T lymphocytes. Human peripheral blood mononuclear cells (PBMC) were prepared by density gradient separation and culture with the presence or absence of CB-MSC. PBMC were collected for activation analysis by flow cytometry at 24-, 48-, and 72- hours. The results showed that, CB-MSC does not stimulate nor inhibit T lymphocyte activation.
间充质干细胞（MSC）的免疫调节特性为基于细胞的新疗法带来了新的见解。然而，最近关于MSC的研究仅集中在骨髓来源的MSC上。我们评估了脐带血来源的MSC（CB-MSC）对T淋巴细胞的免疫原性。通过密度梯度分离制备人外周血单个核细胞（PBMC），并在有或无CB-MSC的情况下进行培养。在24小时、48小时和72小时收集PBMC，通过流式细胞术进行活化分析。结果表明，CB-MSC既不刺激也不抑制T淋巴细胞活化。
