Gasser Paul J, Orchinik Miles, Raju Ilangovan, Lowry Christopher A
Department of Biomedical Sciences, Marquette University, Milwaukee, Wisconsin 53201-1881, USA.
J Comp Neurol. 2009 Feb 1;512(4):529-55. doi: 10.1002/cne.21921.
Organic cation transporter 3 (OCT3) is a high-capacity, low-affinity transporter that mediates bidirectional, sodium-independent transport of dopamine, norepinephrine, epinephrine, serotonin, and histamine. OCT3-mediated transport is directly inhibited by corticosterone, suggesting a potential role for the transporter in mediating some of the effects of stress and glucocorticoids on monoaminergic neurotransmission. To elucidate the importance of OCT3 in clearance of extracellular monoamines in the brain, we used immunohistochemical techniques to describe the distribution of OCT3-like-immunoreactive (OCT3-ir) cells throughout the rostrocaudal extent of adult male rat brains. OCT3-ir cell bodies were widely distributed throughout the brain, with the highest densities observed in the superior and inferior colliculi, islands of Calleja, subiculum, lateral septum, lateral and dorsomedial hypothalamic nuclei, and granule cell layers of the main and accessory olfactory bulbs, the cerebellum, and the retrosplenial granular cortex. OCT3-ir cells and/or fibers were also observed in circumventricular organs, and OCT3-ir ependymal cells were observed in the linings of all cerebral ventricles. The widespread distribution of OCT3-ir cell bodies, including regions receiving dense monoaminergic projections, suggests an important role for this transporter in regulating extracellular concentrations of monoamines in the rat brain and is consistent with the hypothesis that corticosterone-induced inhibition of OCT3-mediated transport may contribute to effects of acute stress or corticosterone on monoaminergic neurotransmission.
有机阳离子转运体3（OCT3）是一种高容量、低亲和力的转运体，介导多巴胺、去甲肾上腺素、肾上腺素、5-羟色胺和组胺的双向、不依赖钠的转运。OCT3介导的转运受到皮质酮的直接抑制，这表明该转运体在介导应激和糖皮质激素对单胺能神经传递的某些影响中可能发挥潜在作用。为了阐明OCT3在清除大脑细胞外单胺中的重要性，我们使用免疫组织化学技术描述了成年雄性大鼠大脑从头尾方向上OCT3样免疫反应性（OCT3-ir）细胞的分布。OCT3-ir细胞体广泛分布于整个大脑，在上下丘、Calleja岛、海马下脚、外侧隔、外侧和背内侧下丘脑核以及主嗅球和副嗅球的颗粒细胞层、小脑和压后颗粒皮层中观察到最高密度。在室周器官中也观察到OCT3-ir细胞和/或纤维，在所有脑室的衬里中观察到OCT3-ir室管膜细胞。OCT3-ir细胞体的广泛分布，包括接受密集单胺能投射的区域，表明该转运体在调节大鼠大脑细胞外单胺浓度方面具有重要作用，这与皮质酮诱导的对OCT3介导转运的抑制可能导致急性应激或皮质酮对单胺能神经传递的影响这一假设一致。
