Xia Chao-Ming, Rong Rong, Lu Zheng-Xian, Shi Chang-Jun, Xu Jing, Zhang Hui-Qin, Gong Wei, Luo Wei
Department of Parasitology, Medical College of Soochow University, Jiangsu Province, PR China.
Exp Parasitol. 2009 Feb;121(2):175-9. doi: 10.1016/j.exppara.2008.10.017. Epub 2008 Nov 7.
A specific PCR assay for the detection of Schistosoma japonicum DNA in rabbit fecal and serum samples was developed by amplifying a 230-bp fragment from the sequence information of the clone G55A of the highly repetitive retrotransposon SjR2. The minimum amount of DNA detectable using the PCR assay was 0.8pg, and the expected PCR product was amplified when DNA equivalent of 1.1 egg from feces was used as template. In the meantime, serum anti-worm IgG was examined by ELISA. ELISA gave positive results at 4-6 weeks post-infection depending on the cercarial doses. The parasite eggs were detected in feces at 7 weeks post-infection. In contrast, S. japonicum DNA was detected in sera at first week post-infection, and it became negative at 10 weeks post-treatment, whereas the anti-worm IgG was still at high levels at 23 weeks post-treatment. These data demonstrated that the PCR assay established provides a potential tool for the early diagnosis and therapy evaluation for S. japonicum infection in humans.
通过从高度重复反转录转座子SjR2的克隆G55A的序列信息中扩增出一个230bp的片段，开发了一种用于检测兔粪便和血清样本中日本血吸虫DNA的特异性PCR检测方法。使用该PCR检测方法可检测到的DNA最小量为0.8pg，当使用相当于1.1个粪便虫卵的DNA作为模板时，可扩增出预期的PCR产物。同时，通过ELISA检测血清抗虫IgG。ELISA在感染后4-6周根据尾蚴剂量给出阳性结果。在感染后7周在粪便中检测到寄生虫卵。相比之下，在感染后第一周在血清中检测到日本血吸虫DNA，在治疗后10周变为阴性，而抗虫IgG在治疗后23周仍处于高水平。这些数据表明，所建立的PCR检测方法为人类日本血吸虫感染的早期诊断和治疗评估提供了一种潜在工具。
