Skehel J M, Pilkington S J, Runswick M J, Fearnley I M, Walker J E
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
FEBS Lett. 1991 Apr 22;282(1):135-8. doi: 10.1016/0014-5793(91)80462-c.
The amino acid sequence of the 10 kDa subunit of the flavoprotein (FP) fragment of complex I from bovine heart mitochondria has been determined by protein sequence analysis, thereby completing the sequence of the FP fragment. The calculated molecular weight of the 10 kDa subunit agrees exactly with the value of 8438 determined by electrospray mass spectrometry, and further confirmation of the sequence has been obtained by sequencing cDNAs amplified from total bovine heart cDNA by the polymerase chain reaction, using mixed oligonucleotides based upon the protein sequence as primers and hybridization probes. The sequence of the 10 kDa subunit is not related to that of any known protein. Being devoid of cysteine residues, it has none of the characteristic features of known iron-sulfur proteins and it is improbable that it is involved in liganding Fe-S centers in the FP fragment.
通过蛋白质序列分析确定了牛心线粒体复合物I黄素蛋白（FP）片段10 kDa亚基的氨基酸序列，从而完成了FP片段的序列测定。10 kDa亚基的计算分子量与电喷雾质谱法测定的8438的值完全一致，并且通过使用基于蛋白质序列的混合寡核苷酸作为引物和杂交探针，对通过聚合酶链反应从牛心总cDNA扩增的cDNA进行测序，进一步证实了该序列。10 kDa亚基的序列与任何已知蛋白质的序列均无关联。由于缺乏半胱氨酸残基，它没有已知铁硫蛋白的任何特征，并且它不太可能参与FP片段中铁硫中心的配位。
