Yamashoji Shiro
Shizuoka Institute of Science and Technology, Fukuroi, Shizuoka 437-8555, Japan.
Anal Biochem. 2009 Feb 1;385(1):115-9. doi: 10.1016/j.ab.2008.10.038. Epub 2008 Nov 5.
In this study, ethanol inhibited the growth and glucose-induced proton release of yeast cells in a dose-dependent manner. On the other hand, ethanol tolerance of menadione-catalyzed luminol luminescence by yeast cells increased with increasing ethanol concentrations in the growth medium. The intracellular reduced-form nicotinamide adenine dinucleotide (NADH) concentration also increased with increasing ethanol concentrations in the medium and was enough to maintain constant menadione-catalyzed luminol luminescence. These facts suggest that the menadione-catalyzed luminol luminescent assay depending on a NADH:quinone reductase and NADH generation system is useful as a new evaluation assay for assessing the vitality of ethanol-stressed yeast cells, whereas the glucose-induced proton release assay is expected to be useful for the evaluation of cell growth under ethanol stress.
在本研究中，乙醇以剂量依赖的方式抑制酵母细胞的生长和葡萄糖诱导的质子释放。另一方面，酵母细胞对甲萘醌催化的鲁米诺发光的乙醇耐受性随着生长培养基中乙醇浓度的增加而增加。细胞内还原型烟酰胺腺嘌呤二核苷酸（NADH）浓度也随着培养基中乙醇浓度的增加而增加，并且足以维持甲萘醌催化的鲁米诺发光恒定。这些事实表明，依赖于NADH：醌还原酶和NADH生成系统的甲萘醌催化的鲁米诺发光测定法可作为评估乙醇胁迫酵母细胞活力的一种新的评估方法，而葡萄糖诱导的质子释放测定法有望用于评估乙醇胁迫下的细胞生长。
