van Dieten J A, de Koning J, van Rees G P
Division of Medical Pharmacology, Sylvius Laboratory, University of Leiden, The Netherlands.
J Endocrinol. 1991 Apr;129(1):27-33. doi: 10.1677/joe.0.1290027.
When pituitary glands from intact female, but not from ovariectomized rats, are incubated for 8 h in medium TC199 without further additives, FSH is synthesized. This LHRH-independent (or autonomous) FSH synthesis is prevented when bovine follicular fluid (bFF) is added to the incubation medium. Results from preliminary experiments, however, indicate no clear autonomous FSH synthesis after long-term absence of LHRH. To investigate the regulatory mechanisms involved in autonomous FSH synthesis and release, pituitary glands (exposed to endogenous LHRH) and pituitary grafts (not exposed to endogenous LHRH) from intact and ovariectomized rats were incubated for 8 h in medium TC199. Total FSH content (FSH released plus FSH remaining in the tissue) was compared with that in non-incubated glands or grafts, giving an indication of FSH synthesis. In addition, some of the animals were given LHRH pulses for 40 h before incubation. When pituitary tissue was taken from intact female rats, FSH synthesis occurred in the animals' own glands and in grafts from LHRH-pretreated rats. No FSH synthesis was seen in ovariectomized rats with or without pretreatment with bFF and/or LHRH. However, when ovariectomized rats had been pretreated with oestrogen, FSH synthesis was measured in vitro after pulsatile LHRH treatment in vivo. The results indicate that autonomous FSH synthesis in vitro is dependent upon previous (in vivo) exposure of the glands to both oestrogen and LHRH.
当完整雌性大鼠而非去卵巢大鼠的垂体在无其他添加剂的TC199培养基中孵育8小时时，促卵泡激素（FSH）会合成。当向孵育培养基中添加牛卵泡液（bFF）时，这种不依赖促性腺激素释放激素（LHRH）（或自主）的FSH合成会受到抑制。然而，初步实验结果表明，在长期缺乏LHRH后，没有明显的自主FSH合成。为了研究自主FSH合成和释放所涉及的调节机制，将完整和去卵巢大鼠的垂体（暴露于内源性LHRH）和垂体移植体（未暴露于内源性LHRH）在TC199培养基中孵育8小时。将总FSH含量（释放的FSH加上组织中剩余的FSH）与未孵育的腺体或移植体中的总FSH含量进行比较，以指示FSH的合成情况。此外，一些动物在孵育前接受了40小时的LHRH脉冲处理。当从完整雌性大鼠获取垂体组织时，FSH在动物自身的腺体以及来自LHRH预处理大鼠的移植体中均有合成。在去卵巢大鼠中，无论是否用bFF和/或LHRH进行预处理，均未观察到FSH合成。然而，当去卵巢大鼠用雌激素进行预处理后，在体内进行脉冲式LHRH处理后，可在体外检测到FSH合成。结果表明，体外自主FSH合成取决于腺体先前（在体内）对雌激素和LHRH的暴露。
