Zhong Yan-Wei, Liang Zhao-Ling, Ren Xiao-Qiang, Li Xiao-Dong, Xu Zhi-Hui, Li Le, Xu Dong-Ping
Viral Hepatitis Research Laboratary, Institute of Infectious Disease of PL4 302 Hospital, Beijing 100039, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Jun;22(3):225-7.
To quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.
Primers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA). The products were amplified by real-time PCR with primers spanning.
The detection rate of serum HBV cccDNA was found to correlate directly with serum HBV DNA loading. HBeAg positive chronic hepatitis B patients had higher serum HBV cccDNA levels than HBeAg negative chronic hepatitis B patients.
The method is good because of the high specificity. It can be used for detection of HBV cccDNA. DNA;
采用新建立的检测方法定量检测慢性乙型肝炎患者血清中的乙型肝炎病毒共价闭合环状DNA（HBV cccDNA）。
在HBV DNA的高度保守区域设计引物和探针。从175例慢性乙型肝炎患者的血清样本中提取DNA，并用质粒安全ATP依赖性核酸酶（PSAD）处理以消除松弛环状DNA（rcDNA）。产物用跨越引物通过实时PCR扩增。
血清HBV cccDNA的检测率与血清HBV DNA载量直接相关。HBeAg阳性慢性乙型肝炎患者的血清HBV cccDNA水平高于HBeAg阴性慢性乙型肝炎患者。
该方法特异性高，效果良好。可用于检测HBV cccDNA。DNA；
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