基于16S-23S rRNA基因内部转录间隔区序列的常见分枝杆菌种内基因型分析
[Analysis of the genotype of intraspecies of common Mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences].
作者信息
Yan Li-Xi, Ju Jun, Chen Bao-Wen, Zhu Bing-Dong, Wang Guo-Zhi
机构信息
Lanzhou Medical College, Lanzhou University, Lanzhou 730000, China.
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 2008 Jun;31(6):436-41.
OBJECTIVE
To analyze the genotypes of intraspecies of common mycobacteria.
METHODS
The genotypes of 94 strains of mycobacteria from DSMZ, as compared with 12 reference strains, were studied by 16S-23S rRNA internal transcription space (ITS) sequence analysis.
RESULTS
The sequencing of 16S-23S rRNA ITS of the 106 strains of common mycobacteria were completed. All the mycobacteria could be discriminated to several genotypes except 4 M. intracellulare, 4 M. avium, 6 M. marinum and 2 M. malmoense which were identical to their reference strains.
CONCLUSIONS
16S-23S rRNA ITS sequence analysis is a reliable method to discriminate mycobacteria in interspecies even in intraspecies.
目的
分析常见分枝杆菌种内的基因型。
方法
采用16S - 23S rRNA内部转录间隔区（ITS）序列分析方法，对来自德国微生物和细胞培养物保藏中心（DSMZ）的94株分枝杆菌菌株的基因型进行研究，并与12株参考菌株进行比较。
结果
完成了106株常见分枝杆菌的16S - 23S rRNA ITS测序。除4株胞内分枝杆菌、4株鸟分枝杆菌、6株海分枝杆菌和2株马尔默分枝杆菌与各自的参考菌株相同外，所有分枝杆菌均可分为几种基因型。
结论
16S - 23S rRNA ITS序列分析是一种可靠的方法，即使在种内也能鉴别分枝杆菌。
Zotero 插件