[骨桥蛋白在大鼠非酒精性脂肪性肝纤维化发生发展中的表达及意义]

[The expression and significance of osteopontin in the development of nonalcoholic fatty liver fibrosis in rats].

作者信息

Liu Ping, Chen Dong-feng, Wang Jun, Ai Zheng-lin, Fan Li-lin, Wang Xiao-min, Yan Li-li

机构信息

Department of Gastroenterology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2008 Nov;16(11):831-4.

PMID:19032867

Abstract

OBJECTIVE

To investigate the changes of osteopontin (OPN) in the liver tissues during nonalcoholic fatty liver fibrosis in rats and to explore the effect of OPN in the development of nonalcoholic fatty liver fibrosis.

METHODS

Fifty-six male Wistar rats were randomly divided into a control group (8 rats) and a high-fat diet group. The high-fat diet group was divided into 6 subgroups (8 rats in each subgroup) with high-fat feedings for 4, 8, 12, 16, 20 or 24 weeks. Conventional histochemical, HE, Masson-trichrome and immunohistochemical staining for alpha-smooth muscle actin (a-SMA) were performed with the liver histological preparations. The expression of OPN was detected with reverse transcription and polymerase chain reactions and Western blot.

RESULTS

Levels of OPN in liver tissues in rat nonalcoholic fatty liver fibrosis induced by high-fat diet were significantly increased over those in the control group (F=7.15, P less than 0.01). OPN expressions were closely correlated with a-SMA and nonalcoholic fatty liver fibrosis, and correlation coefficients of the two groups were 0.94 and 0.82, and both P values were less than 0.01.

CONCLUSION

Expression of OPN increases dramatically in the livers during the development of nonalcoholic fatty liver fibrosis, and OPN may play an important role in this event.

摘要

目的

研究大鼠非酒精性脂肪肝纤维化过程中肝组织骨桥蛋白（OPN）的变化，探讨OPN在非酒精性脂肪肝纤维化发生发展中的作用。

方法

将56只雄性Wistar大鼠随机分为对照组（8只）和高脂饮食组。高脂饮食组再分为6个亚组（每组8只），分别给予高脂饲料喂养4、8、12、16、20或24周。对肝脏组织标本进行常规组织化学、HE、Masson三色染色及α-平滑肌肌动蛋白（α-SMA）免疫组织化学染色。采用逆转录聚合酶链反应和蛋白质免疫印迹法检测OPN的表达。