Ammari R, Lopez C, Fiorentino H, Gonon F, Hammond C
Institut de Neurobiologie de la Méditerranée, U901 Inserm-Université de la Méditerranée, 163 route de Luminy, 13273 Marseille, France.
Neuroscience. 2009 Mar 3;159(1):3-6. doi: 10.1016/j.neuroscience.2008.10.051. Epub 2008 Nov 7.
The effect of endogenous dopamine on the activity of target neurons recorded with patch clamp or Ca2+ imaging techniques in slices has been studied to date with intra-striatal stimuli. Yet, this approach is severely handicapped by the nonphysiological and nonspecific stimulation of local neurons and fibers within the striatum. We now report a new juvenile and adult mouse slice preparation in which a component of the nigro-striatal dopaminergic pathway is preserved in its entirety, from cell bodies to axon terminals. This tilted parasagittal slice (380-400 microm) just medial to the subthalamic nucleus contains functional nigro-striatal neurons as assessed by morphological examination of tyrosine hydroxylase positive cell bodies and axons, combined with electrochemical assays of dopamine release in the striatum in response to stimulation of the substantia nigra pars compacta. The nigro-striatal slice constitutes a suitable in vitro preparation to determine the impact of endogenously released dopamine on target neurons of the striatum.
迄今为止，利用脑片内纹状体刺激，研究了内源性多巴胺对采用膜片钳或Ca2+成像技术记录的脑片靶神经元活性的影响。然而，这种方法受到纹状体内局部神经元和纤维非生理性和非特异性刺激的严重限制。我们现在报告一种新的幼年和成年小鼠脑片制备方法，其中黑质-纹状体多巴胺能通路的一个组成部分从细胞体到轴突终末都完整保留。这种倾斜的矢状旁切片（380-400微米）位于丘脑底核内侧，通过酪氨酸羟化酶阳性细胞体和轴突的形态学检查，结合对黑质致密部刺激后纹状体内多巴胺释放的电化学分析，评估其包含功能性黑质-纹状体神经元。黑质-纹状体脑片构成了一种合适的体外制备方法，用于确定内源性释放的多巴胺对纹状体靶神经元的影响。
