Aubry Karine, Shao Ziyang, Monteil Jacques, Paraf François, Bessède Jean Pierre, Rigaud Michel
Department of E.N.T., University Hospital Center, 2 Avenue Martin Luther-King, 87000, Limoges, France.
Mol Imaging Biol. 2009 Mar-Apr;11(2):88-93. doi: 10.1007/s11307-008-0183-y. Epub 2008 Nov 25.
The aim of this study was to demonstrate the ability to use human clinical positron emission tomography/computed tomography (PET/CT) to detect and investigate head and neck cancers chemically induced by 4-nitroquinoline-1-oxide (4-NQO) in a rat model.
The study design was prospective animal research.
A head and neck squamous cell carcinoma was established in 20 immunocompetent rats, who drank a 4-NQO solution during 16 weeks. 2-Deoxy-2-[F-18]fluoro-D: -glucose (FDG)-PET/CT was performed for five of them, 34 weeks after the start of the experiment to characterize the tumors. A day following the FDG-PET/CT, rats were euthanized and pathological features were evaluated by hematoxylin-eosin staining.
All rats had head and neck tumor at various locations at 34 weeks. Among the five rats selected for having FDG-PET/CT, the clinical examination detected exophytic tumors grown in the oral cavity for three of them (one on the inferior lip, one on the hard palate, and one on the internal side of the cheek). FDG-PET/CT confirmed the presence of those tumors and detected ones located on the base of tongue for three of them. Tumor extensions were characterized and tumor metabolic volumes were measured. The smallest lesion detected measured 3 x 3 x 4 mm. Pathologic examination using hematoxylin-eosin staining confirmed squamous cell carcinoma.
This study demonstrated that FDG-PET/CT is a feasible examination to detect occult primary tumors in rat models. It is useful to follow tumor progression and evaluate therapeutics efficacy.
本研究旨在证明利用人体临床正电子发射断层扫描/计算机断层扫描（PET/CT）检测和研究大鼠模型中由4-硝基喹啉-1-氧化物（4-NQO）化学诱导的头颈癌的能力。
研究设计为前瞻性动物研究。
在20只具有免疫活性的大鼠中建立头颈鳞状细胞癌模型，这些大鼠在16周内饮用4-NQO溶液。实验开始34周后，对其中5只大鼠进行2-脱氧-2-[F-18]氟-D-葡萄糖（FDG）-PET/CT检查以对肿瘤进行特征描述。在FDG-PET/CT检查后的一天，对大鼠实施安乐死，并通过苏木精-伊红染色评估病理特征。
在34周时，所有大鼠在不同部位均出现头颈肿瘤。在被选进行FDG-PET/CT检查的5只大鼠中，临床检查发现其中3只大鼠口腔内有外生性肿瘤生长（1只在下唇，1只在硬腭，1只在脸颊内侧）。FDG-PET/CT证实了这些肿瘤的存在，并检测到其中3只大鼠舌根部有肿瘤。对肿瘤的扩展情况进行了特征描述，并测量了肿瘤代谢体积。检测到的最小病变尺寸为3×3×4毫米。苏木精-伊红染色的病理检查证实为鳞状细胞癌。
本研究表明，FDG-PET/CT是检测大鼠模型中隐匿性原发性肿瘤的一种可行检查方法。它有助于跟踪肿瘤进展并评估治疗效果。
