Chen Xiao-Guang, Bai Tao, Wu Bin-Yang, Wang Jun-Ke
Department of Anesthesiology, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Zhonghua Yi Xue Za Zhi. 2008 Jun 17;88(23):1648-50.
To investigate the effects of glutamine on the changes of nuclear factor (NF)-kappaB and lung pathology in acute lung injury.
Forty SD rats underwent injection of lipopolysaccharide (LPS) 5 mg/kg into the femoral vein, and were randomly divided into 4 equal groups: Group B: undergoing injection of glutamine 0.75 g/kg into the femoral vein 1 h before LPS injection, Group C undergoing injection of glutamine and LPS simultaneously, and Group D undergoing injection of glutamine 1 h after LPS injection, and Group E without glutamine injection. Another 10 rats underwent injection of normal saline to be used as controls (Group A). Four hours after the LPS injection the rats were killed with their lungs taken out RT-PCR was used to detect the level of nuclear factor (NF)-kappaB mRNA expression. ELISA was used to detect the tumor necrosis factor (TNF) - in lung. The activity of superoxide dismutase (SOD) was examined by hydroxylamine method, and the malondialdehyde level was determined by thiobarbituric acid (TBA) method.
The lung NF-kappaB mRNA expression levels and TNF- levels of Groups B, C, D, and E were all significantly higher than that of Group A (all P < 0.01). The lung NF-kappaB mRNA expression levels and TNF- levels of Groups C and D were all significantly lower than those of Group B (P < 0.01). However, there were no significant differences in lung NF-kappaB mRNA expression level and TNF- level between Group B and Group E (both P > 0.05). The SOD activity of Group B was significantly lower than that of Group A and the MDA content of Group B was significantly higher than that of Group A (both P < 0.05). The SOD activity levels of Groups C and D were significantly higher than that of Group B and the MDA content of Groups C and D were significantly lower than that of Group B (P < 0.01 or P < 0.05). However there were no significant differences in lung SDD activity and MDA content between Groups B and E (both P > 0.05). Obvious inflammatory changes were seen in the lungs of Groups B and E at the similar extent. Only slight infiltration could be seen in Groups C and D. The lung of Group A was normal.
Early glutamine administration protects the lung against acute LPS injury. The mechanism may be inhibition of the overexpression of NF-kappaB mRNA.
探讨谷氨酰胺对急性肺损伤中核因子（NF）-κB变化及肺病理的影响。
40只SD大鼠经股静脉注射5mg/kg脂多糖（LPS），随机分为4组，每组10只：B组：在注射LPS前1小时经股静脉注射0.75g/kg谷氨酰胺；C组：同时注射谷氨酰胺和LPS；D组：在注射LPS后1小时注射谷氨酰胺；E组：不注射谷氨酰胺。另取10只大鼠注射生理盐水作为对照组（A组）。注射LPS 4小时后处死大鼠，取出肺组织，采用RT-PCR检测核因子（NF）-κB mRNA表达水平，ELISA检测肺组织中肿瘤坏死因子（TNF）-α水平，用羟胺法检测超氧化物歧化酶（SOD）活性，硫代巴比妥酸（TBA）法测定丙二醛水平。
B、C、D、E组肺组织NF-κB mRNA表达水平和TNF-α水平均显著高于A组（均P<0.01）。C组和D组肺组织NF-κB mRNA表达水平和TNF-α水平均显著低于B组（P<0.01）。但B组与E组肺组织NF-κB mRNA表达水平和TNF-α水平比较差异无统计学意义（均P>0.05）。B组SOD活性显著低于A组，B组丙二醛含量显著高于A组（均P<0.05）。C组和D组SOD活性水平显著高于B组，C组和D组丙二醛含量显著低于B组（P<0.01或P<0.05）。但B组与E组肺组织SDD活性和丙二醛含量比较差异无统计学意义（均P>0.05）。B组和E组肺组织可见明显程度相似的炎症改变。C组和D组仅见轻微浸润。A组肺组织正常。
早期给予谷氨酰胺可保护肺组织免受急性LPS损伤。其机制可能是抑制NF-κB mRNA的过度表达。
