Chen Yun-Zhao, Zhou Xiao-Ge, Jin Yan, Zheng Yuan-Yuan, Chen Gang, Shi Yan
Department of Pathology, Beijing Friendship Hospital, Affiliated to Capital Medical University, Beijing 100050, China.
Zhonghua Bing Li Xue Za Zhi. 2008 Jul;37(7):440-4.
To study the clinical and morphological features, immunophenotype and in situ detection of Epstein-Barr virus (EBV) infection in infectious mononucleosis (IM) to enhance the knowledge and diagnosis of the disease.
Using routine haematoxylin and eosin staining, immunohistochemistry and EBER in situ hyhridization together with clinical data analysis, 15 cases of IM were evaluated for their clinical features, morphology, immunophenotype and EBV infection status.
IM was common in children and young adults with a median age of 18 years. It was an acute disease with lymphadenopathy and frequently fever. Most of the patients had a rapid recovery. Every case showed a markedly T zone expansion with a mottling pattern, composing of small to large lymphocytes, plasma cells and histiocytes. The cells also showed a B-cell differentiation profile ranging from activated lymphoblastoid cells, immunoblasts, plasmablasts, plasma-like cells and plasma cells. Many small lymphocytes in the expanded T zone expressed CD3. Some of the activated lymphoblastoid cells and immonoblasts were CD20 and CD30 positive with variable intensity signals. EBER positive (nuclear staining) cells were seen in every case. The number of EBER positive cells ranged from 10 to more than 100 per high power field. These cells included small to large lymphocytes locating mostly in the expanded T zone and a few were in the follicular germinal centers.
IM is an EBV related acute sell-recovering lymphoproliferative disease, having distinct clinical, morphological and immunophenotypic characteristics as well as EBV infection. Taking these features into consideration will facilitate the correct diagnosis of IM.
研究传染性单核细胞增多症（IM）的临床和形态学特征、免疫表型以及爱泼斯坦-巴尔病毒（EBV）感染的原位检测，以增进对该疾病的认识和诊断。
采用常规苏木精和伊红染色、免疫组织化学及EBER原位杂交，并结合临床数据分析，对15例IM患者的临床特征、形态学、免疫表型及EBV感染状况进行评估。
IM常见于儿童和青年，中位年龄为18岁。它是一种伴有淋巴结病且常发热的急性疾病。大多数患者恢复迅速。每例均显示T区明显扩大，呈斑驳状，由小至大淋巴细胞、浆细胞和组织细胞组成。细胞还呈现出从活化淋巴母细胞、免疫母细胞、浆母细胞、浆样细胞到浆细胞的B细胞分化谱。扩大的T区许多小淋巴细胞表达CD3。一些活化淋巴母细胞和免疫母细胞CD20和CD30呈阳性，信号强度不一。每例均可见EBER阳性（核染色）细胞。每个高倍视野中EBER阳性细胞数量为10至100多个。这些细胞包括小至大淋巴细胞，大多位于扩大的T区，少数位于滤泡生发中心。
IM是一种与EBV相关的急性自限性淋巴细胞增殖性疾病，具有独特的临床、形态学和免疫表型特征以及EBV感染。考虑这些特征将有助于IM的正确诊断。
