Shi Jie, Lu Zhao-Hui, Cui Quan-Cai
Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Bing Li Xue Za Zhi. 2008 Jul;37(7):465-70.
To study the proteomics of papillary thyroid carcinoma (PTC), and to detect the differential expression of proteins compared to normal thyroid (NT) tissue.
Protein extracts of papillary thyroid carcinoma and normal thyroid tissues from 10 patients were separated by proteomics technique and identified using Image Master software. The selected differential proteins were sent to Proteomics Research Center of Chinese Academy of Medical Sciences for mass-spectrometry identification.
The basic 2-dimensional electrophoresis (2-DE) images of PTC and NT were obtained, and four potential differential expression spots were selected for mass-spectrometry identification. Among them, annexin I, peroxiredoxin I, mitochondrial aconitase were up-regulated while carbonic anhydrase I was down-regulated.
Proteomics may provide a new direction to study PTC, by identifying some tumor-specific markers, leading to elucidation of the mechanism of tumorigenesis and identification of therapeutic targets.
研究甲状腺乳头状癌（PTC）的蛋白质组学，并检测与正常甲状腺（NT）组织相比蛋白质的差异表达。
采用蛋白质组学技术分离10例患者的甲状腺乳头状癌和正常甲状腺组织的蛋白质提取物，并使用Image Master软件进行鉴定。将筛选出的差异蛋白送至中国医学科学院蛋白质组学研究中心进行质谱鉴定。
获得了PTC和NT的基本二维电泳（2-DE）图像，选择了4个潜在的差异表达点进行质谱鉴定。其中，膜联蛋白I、过氧化物氧还蛋白I、线粒体乌头酸酶上调，而碳酸酐酶I下调。
蛋白质组学通过鉴定一些肿瘤特异性标志物，可能为研究PTC提供新的方向，从而阐明肿瘤发生机制并确定治疗靶点。
