Luo Chang-ying, Li Ben-shang, Jiang Hua, Gu Long-jun
Department of Hematology & Oncology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Zhonghua Xue Ye Xue Za Zhi. 2008 Jul;29(7):446-9.
To determine whether the high level of asparagine synthetase (AS) expression in childhood acute lymphocytic leukemia (ALL) is associated with an inferior prognosis.
AS mRNA level in leukemic cells from 53 newly diagnosed ALL children was measured by real time fluorescent quantitative PCR method. Patients were divided into groups according to their relapse risk and outcome, and the AS expression levels in each group were compared. The survival rates in different AS expressing level groups were estimated and compared.
The highest level of AS [median 17.25 (2.48-46. 82)] was observed in children failed remission, intermediate level [14.28 (3.20-54.47)] in relapsed children and the lowest level [5.08 (0.84-54.92)] in children with continuous complete remission (CCR) (P<0.05). The AS mRNA level [14.93 (2.48-54.47)] in children with poor outcome (un-remission and relapsed) was significantly higher than that in children in CCR (P<0.01). The two-year estimated disease free survival was much lower in children with high AS expression (53.8%) than in those with low AS expression (84.6%) (P<0.05).
High expression of AS is associated with a poor outcome in ALL children.
确定儿童急性淋巴细胞白血病（ALL）中高水平的天冬酰胺合成酶（AS）表达是否与较差的预后相关。
采用实时荧光定量PCR法检测53例新诊断ALL患儿白血病细胞中的AS mRNA水平。根据复发风险和转归将患者分组，比较各组AS表达水平。评估并比较不同AS表达水平组的生存率。
未缓解患儿的AS水平最高[中位数17.25（2.48 - 46.82）]，复发患儿为中等水平[14.28（3.20 - 54.47）]，持续完全缓解（CCR）患儿最低[5.08（0.84 - 54.92）]（P<0.05）。预后不良（未缓解和复发）患儿的AS mRNA水平[14.93（2.48 - 54.47）]显著高于CCR患儿（P<0.01）。AS高表达患儿的两年无病生存率（53.8%）远低于低表达患儿（84.6%）（P<0.05）。
ALL患儿中AS高表达与不良预后相关。
