Li Lei, Xiao Qiang, Xie Yu-bo, Ma Yu-lin, Tang Zhen-yong, Yin Yong-shuo
Department of Surgery, the First Hospital Affiliated to Guangxi Medical University, Nanning 530021, China.
Zhonghua Wai Ke Za Zhi. 2008 Jun 1;46(11):847-50.
To investigate the effect of human caudal-related homeobox 2 (Cdx2) gene expression on human gastric carcinoma cell line MGC-803.
pCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 cells were divided into 4 groups: non-transfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA. Cdx2 gene and its protein expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot techniques respectively. The effects of Cdx2 overexpression on the growth of MGC-803 cells in vitro was assessed by measuring MTT, flow cytometry and transmission electron microscopy analysis.
In MGC-803 cells transfected with pCMV-Cdx2-HA, cell proliferation was significantly suppressed, the cells was ultrastructurally destroyed, cell cycle progression was blocked in G0/G1 and apoptosis rate was higher (P<0.05) in comparison with non-transfected cells or cells transfected with empty vector.
It indicated that Cdx2 gene can suppress the growth of gastric carcinoma and may save as a novel therapeutic target.
研究人类尾型相关同源盒基因2（Cdx2）表达对人胃癌细胞系MGC-803的影响。
采用DNA重组方法构建pCMV-Cdx2-HA真核表达质粒。将MGC-803细胞分为4组：未转染组、转染pCMV-HA组、转染pCMV-GAPDH-HA组、转染pCMV-Cdx2-HA组。分别采用实时定量聚合酶链反应（qRT-PCR）和蛋白质免疫印迹技术检测Cdx2基因及其蛋白表达。通过MTT法、流式细胞术和透射电镜分析评估Cdx2过表达对MGC-803细胞体外生长的影响。
与未转染细胞或转染空载体的细胞相比，转染pCMV-Cdx2-HA的MGC-803细胞增殖明显受到抑制，细胞超微结构被破坏，细胞周期进程阻滞于G0/G1期，凋亡率更高（P<0.05）。
表明Cdx2基因可抑制胃癌生长，可能成为一种新的治疗靶点。
