Palard Mickaël, Combes Julien, Champion Eric, Foucaud Sylvie, Rattner Aline, Bernache-Assollant Didier
Université de Limoges, CNRS, SPCTS UMR 6638, Faculté des Sciences et Techniques, 123 Avenue Albert Thomas, 87060 Limoges Cedex, France.
Acta Biomater. 2009 May;5(4):1223-32. doi: 10.1016/j.actbio.2008.10.016. Epub 2008 Nov 6.
Silicated hydroxyapatite powders (Ca10(PO4)(6-x)(SiO4)x(OH)(2-x); Si(x)HA) were synthesized using a wet precipitation method. The sintering of Si(x)HA ceramics with 0 < or = x < or = 1 was investigated. For 0 < or = x < or = 0.5, the sintering rate and grain growth decreased slightly with the amount of silicate. For larger amounts, the sintering behaviour differed with the formation of secondary phases before total densification. Sintering parameters (temperature and time) were adjusted to each composition to produce dense materials having similar microstructure without formation of these secondary phases. Dense ceramics made of pure hydroxyapatite and Si(x)HA containing various amounts of silicate (up to x = 0.6) were biologically tested in vitro with human osteoblast-like cells. The proliferation of cells on the surface of the ceramics increased up to 5 days of culture, indicating that the materials were biocompatible. However, the silicon content did not influence the cell proliferation.
采用湿沉淀法合成了硅羟基磷灰石粉末（Ca10(PO4)(6 - x)(SiO4)x(OH)(2 - x)；Si(x)HA）。研究了0≤x≤1的Si(x)HA陶瓷的烧结情况。对于0≤x≤0.5，烧结速率和晶粒生长随硅酸盐含量的增加略有下降。对于更高的含量，烧结行为因在完全致密化之前形成第二相而有所不同。针对每种成分调整烧结参数（温度和时间），以制备具有相似微观结构且不形成这些第二相的致密材料。用人类成骨样细胞对由纯羟基磷灰石和含有不同硅酸盐含量（高达x = 0.6）的Si(x)HA制成的致密陶瓷进行了体外生物学测试。在培养5天内，陶瓷表面细胞的增殖增加，表明这些材料具有生物相容性。然而，硅含量并不影响细胞增殖。
