嗜热脂肪芽孢杆菌染色体α-淀粉酶基因的克隆
Cloning of a chromosomal alpha-amylase gene from Bacillus stearothermophilus.
作者信息
Jørgensen P L, Poulsen G B, Diderichsen B
机构信息
Novo Nordisk, Bagsvoerd, Denmark.
出版信息
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):271-5. doi: 10.1016/0378-1097(91)90564-q.
PMID:1903751
Abstract
We have cloned and sequenced a gene for a heat-stable alpha-amylase from a natural isolate of Bacillus stearothermophilus. Previously, it had been shown that B. stearothermophilus amylase genes may be harboured on indigenous plasmids. We have found that our isolate harbours the amylase gene only on the chromosome and not on its indigenous plasmid.
摘要
我们已经从嗜热脂肪芽孢杆菌的一个天然分离株中克隆并测序了一个热稳定α-淀粉酶基因。此前已表明，嗜热脂肪芽孢杆菌淀粉酶基因可能存在于内源质粒上。我们发现我们的分离株仅在染色体上而非内源质粒上含有淀粉酶基因。
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