Stoll Sascha, Feldhaar Heike, Gross Roy
Lehrstuhl für Mikrobiologie, Biozentrum, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
Environ Microbiol. 2009 Apr;11(4):877-88. doi: 10.1111/j.1462-2920.2008.01808.x. Epub 2008 Nov 10.
The transcriptome of Blochmannia floridanus, the endosymbiont of the carpenter ant Camponotus floridanus, is presented during various developmental stages of its holometabolous host by use of a whole-genome DNA macroarray. The detected transcription patterns indicate the presence of local transcription units as well as global regulatory mechanisms. Yet, the overall regulation scale is very modest, rarely exceeding a factor of three. A large number of genes show differential expression in different life stages and a distinct expression pattern of genes possibly involved in symbiotic function as compared with housekeeping genes is apparent. However, these transcriptional changes are small as compared with the changes in the number of bacteria during host development, which is the highest in pupae and in young imagines. Control of replication of the bacteria in certain life stages may therefore be the decisive parameter influencing the overall level of gene expression of Blochmannia in the animal. The few highly expressed genes like those encoding molecular chaperones exhibit a significantly higher G+C content than moderately expressed genes.
通过使用全基因组DNA宏阵列，展示了佛罗里达弓背蚁（Camponotus floridanus）的内共生菌——佛罗里达布洛赫曼氏菌（Blochmannia floridanus）在其全变态宿主不同发育阶段的转录组。检测到的转录模式表明存在局部转录单元以及全局调控机制。然而，整体调控规模非常适度，很少超过三倍。大量基因在不同生命阶段表现出差异表达，并且与管家基因相比，可能参与共生功能的基因具有明显不同的表达模式。然而，与宿主发育过程中细菌数量的变化相比，这些转录变化较小，细菌数量在蛹期和年轻成虫期最高。因此，在某些生命阶段控制细菌的复制可能是影响动物体内布洛赫曼氏菌基因表达总体水平的决定性参数。少数高表达基因，如编码分子伴侣的基因，其G+C含量明显高于中度表达的基因。
