Koppenaal D W, Tijssen A M, van Dieten J A, de Koning J
Division of Medical Pharmacology, Center for Bio-Pharmaceutical Sciences, Leiden, The Netherlands.
J Endocrinol. 1991 May;129(2):205-11. doi: 10.1677/joe.0.1290205.
Female rats were treated with Metrodin (highly purified urinary FSH from menopausal women) or saline during the oestrous cycle. On the day of pro-oestrus they were anaesthesized with phenobarbital and received four repetitive LHRH injections 1 h apart. This treatment with FSH suppressed the unprimed LH response to the first LHRH injection. During the subsequent injections the maximal LHRH self-priming was delayed by 3 h till the fourth LHRH stimulation. At this time, LH release in response to LHRH was equally as high as shown in the saline controls after the second LHRH injection. Ovariectomized rats did not show the self-priming effect and FSH treatment was ineffective in suppressing LHRH-induced LH release. Administration of FSH followed by an additional 4- or 24-h period before LHRH stimulation were equally effective in suppressing the unprimed LH release and delaying (up to 3 h) the maximal priming of LH release by LHRH. Even 4-20-fold increased amounts of LHRH did not affect the suppressed unprimed release of LH after FSH treatment. Treatment with FSH did not change oestradiol and progesterone levels. It was concluded that FSH treatment suppresses the unprimed LHRH-induced LH release and delays maximal LHRH self-priming by enhancing the release of an ovarian factor.
在发情周期中，对雌性大鼠用美曲膦酯（从绝经后妇女尿液中高度纯化的促卵泡激素）或生理盐水进行处理。在动情前期那天，用苯巴比妥对它们进行麻醉，并每隔1小时给予4次重复的促性腺激素释放激素（LHRH）注射。这种促卵泡激素处理抑制了对首次LHRH注射的未预处理促黄体生成素（LH）反应。在随后的注射过程中，最大的LHRH自身预处理被延迟3小时，直到第四次LHRH刺激。此时，对LHRH的LH释放与第二次LHRH注射后生理盐水对照组所显示的一样高。去卵巢大鼠未表现出自身预处理效应，促卵泡激素处理在抑制LHRH诱导的LH释放方面无效。在LHRH刺激前给予促卵泡激素，随后再经过4小时或24小时，在抑制未预处理的LH释放和延迟（长达3小时）LHRH对LH释放的最大预处理方面同样有效。即使LHRH量增加4至20倍，也不影响促卵泡激素处理后被抑制的未预处理LH释放。促卵泡激素处理未改变雌二醇和孕酮水平。得出的结论是，促卵泡激素处理通过增强一种卵巢因子的释放来抑制未预处理的LHRH诱导的LH释放，并延迟最大的LHRH自身预处理。
