Denardi Sandra Eloisi, Bechara Gervásio Henrique, Camargo-Mathias Maria Izabel
Departamento de Biologia, I.B., UNESP, Av. 24 A, no 1515, Cx. Postal 199, CEP 13506-900, Rio Claro, SP, Brazil.
Exp Parasitol. 2009 Mar;121(3):213-8. doi: 10.1016/j.exppara.2008.10.019. Epub 2008 Nov 11.
During the process of Arthropoda reproduction, the synthesis and uptake of proteins, carbohydrates, and lipids by oocytes is termed vitellogenesis. These compounds that will make up the yolk may be in ticks endogenously synthesized by the oocytes and/or exogenously produced by the fat body and pedicel cells. This study examined the fat body of Amblyomma cajennense ticks at the cytochemical ultrastructural level to demonstrate the presence of lipids, proteins and carbohydrates in trophocytes. The lipids were detected in higher quantity than proteins and carbohydrates in the fat body cells, suggesting that the role of the fat body in tick is stored lipids and carbohydrates to convert them in energy, or still they could be used with cell structural purpose. The electrophoresis technique applied at A. cajennense fat body demonstrated specifically the molecular mass of proteins: about 98kDa. By the other hands, the fat body is not the organ responsible for the synthesis of the yolk protein, role probably performed by the pedicel cells.
在节肢动物的繁殖过程中，卵母细胞对蛋白质、碳水化合物和脂质的合成与摄取被称为卵黄发生。这些构成卵黄的化合物在蜱类中可能由卵母细胞内源性合成和/或由脂肪体和柄细胞外源性产生。本研究在细胞化学超微结构水平上检查了卡延钝缘蜱的脂肪体，以证明滋养细胞中脂质、蛋白质和碳水化合物的存在。脂肪体细胞中检测到的脂质比蛋白质和碳水化合物的含量更高，这表明蜱类脂肪体的作用是储存脂质和碳水化合物以将它们转化为能量，或者它们仍可用于细胞结构目的。应用于卡延钝缘蜱脂肪体的电泳技术明确显示了蛋白质的分子量：约98kDa。另一方面，脂肪体不是负责卵黄蛋白合成的器官，这一作用可能由柄细胞执行。
