Ramazanova Anna S, Starkov Vladislav G, Osipov Alexey V, Ziganshin Rustam H, Filkin Sergey Yu, Tsetlin Victor I, Utkin Yuri N
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Laboratory of Neuropeptide Receptors, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Toxicon. 2009 Jan;53(1):162-8. doi: 10.1016/j.toxicon.2008.11.001. Epub 2008 Nov 12.
Cysteine-rich proteins found in animal venoms (CRISP-Vs) are members of a large family of cysteine-rich secretory proteins (CRISPs). CRISP-Vs acting on different ion channels were found in venoms or mRNA (cDNA) encoding CRISP-Vs were cloned from snakes of three main families (Elapidae, Colubridae and Viperidae). About thirty snake CRISP-Vs were sequenced so far, however no complete sequence for CRISP-V from Viperinae subfamily was reported. We have cloned and sequenced for the first time cDNAs encoding CRISP-Vs from Vipera nikolskii and Vipera berus vipers (Viperinae). The deduced mature CRISP-V amino acid sequences consist of 220 amino acid residues. Phylogenetic analysis showed that viper proteins are closely related to those of Crotalinae snakes. The presence of CRISP-V in the V. berus venom was revealed using a combination of gel-filtration chromatography, electrophoresis and MALDI mass spectrometry. The finding of the putative channel blocker in viper venom may indicate its action on prey nervous system.
在动物毒液中发现的富含半胱氨酸的蛋白质（CRISP-Vs）是富含半胱氨酸的分泌蛋白（CRISPs）大家族的成员。在毒液中发现了作用于不同离子通道的CRISP-Vs，或者从三个主要科（眼镜蛇科、游蛇科和蝰蛇科）的蛇类中克隆出了编码CRISP-Vs的mRNA（cDNA）。到目前为止，已经对大约30种蛇的CRISP-Vs进行了测序，然而，关于蝰蛇亚科CRISP-V的完整序列尚未见报道。我们首次从极北蝰和贝氏蝰蛇（蝰蛇亚科）中克隆并测序了编码CRISP-Vs的cDNA。推导的成熟CRISP-V氨基酸序列由220个氨基酸残基组成。系统发育分析表明，蝰蛇蛋白与蝮亚科蛇类的蛋白密切相关。通过凝胶过滤色谱、电泳和基质辅助激光解吸电离质谱联用技术，揭示了贝氏蝰蛇毒液中存在CRISP-V。在蝰蛇毒液中发现推定的通道阻滞剂可能表明其对猎物神经系统的作用。
