Satoh Kanako, Nonaka Ryouichi
Department of Environmental Health and Toxicology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Yakugaku Zasshi. 2008 Dec;128(12):1771-82. doi: 10.1248/yakushi.128.1771.
The Tokyo Metropolitan Government in Japan enacted an "Ordinance concerning the abuse prevention of the psychoactive drugs" in April 2006 that prohibited the manufacture, cultivation, sales, possession, use, etc., of these drugs. Therefore, we constructed a reproducible, simple, and small-scale determination method of the psychoactive drugs for the re-uptake and the release of monoamines (dopamine, serotonin and norepinephrine), and the activation of [(35)S]guanosine-5'-O-(3-thio)-triphosphate binding to guanine nucleotide-binding proteins (G proteins). These assays were then applied to study the effects of different kinds (phenethylamine derivatives, tryptamine derivatives, and piperazine derivatives) of non-medically used psychoactive drugs on monoamine re-uptake and release, and G-protein binding. The results suggested that some drugs strongly act on the central nerve system to the same extent as the drugs. This assay system was able to designate psychoactive drugs as prohibited substances in accordance with criteria set forth by the Tokyo Metropolitan government.
日本东京都政府于2006年4月颁布了一项“关于预防精神活性药物滥用的条例”，该条例禁止制造、种植、销售、持有、使用等这些药物。因此，我们构建了一种可重复、简单且小规模的精神活性药物测定方法，用于研究单胺（多巴胺、5-羟色胺和去甲肾上腺素）的再摄取和释放，以及[（35）S]鸟苷-5'-O-（3-硫代）-三磷酸与鸟嘌呤核苷酸结合蛋白（G蛋白）结合的激活情况。然后将这些测定方法应用于研究不同种类（苯乙胺衍生物、色胺衍生物和哌嗪衍生物）的非医用精神活性药物对单胺再摄取和释放以及G蛋白结合的影响。结果表明，一些药物对中枢神经系统的作用与这些药物的作用程度相当。该测定系统能够根据东京都政府规定的标准将精神活性药物指定为违禁物质。
