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用于测量牛肉中肌内脂肪含量的高通量无损核磁共振方法。

High-throughput non-destructive nuclear magnetic resonance method to measure intramuscular fat content in beef.

作者信息

Corrêa Cátia Crispilho, Forato Lucimara Aparecida, Colnago Luiz Alberto

机构信息

Embrapa Instrumentação Agropecuária, Rua XV de Novembro 1452, São Carlos, São Paulo, 13560-970, Brazil.

出版信息

Anal Bioanal Chem. 2009 Feb;393(4):1357-60. doi: 10.1007/s00216-008-2526-6. Epub 2008 Nov 30.

Abstract

High intake of saturated fat from meats has been associated with cardiovascular disease, cancer, diabetes, and others diseases. In this paper, we are introducing a simple, high-throughput, and non-destructive low-resolution nuclear magnetic resonance method that has the potential to analyze the intramuscular fat content (IMF) in more than 1,000 beef portions per hour. The results can be used in nutritional fact labels, replacing the currently used average value. The method is based on longitudinal (T(1)) and transverse (T(2)) relaxation time information obtained by a continuous wave-free precession (CWFP) sequence. CWFP yields a higher correlation coefficient (r = 0.9) than the conventional Carr-Purcell-Meiboom-Gill (CPMG) method (r = -0.25) for IMF in beef and is just as fast and a simpler pulse sequence than CPMG. The method can also be applied to other meat products.

摘要

来自肉类的高饱和脂肪摄入量与心血管疾病、癌症、糖尿病及其他疾病有关。在本文中,我们介绍了一种简单、高通量且无损的低分辨率核磁共振方法,该方法有潜力每小时分析1000多个牛肉切片中的肌内脂肪含量(IMF)。其结果可用于营养成分标签,取代目前使用的平均值。该方法基于通过连续波自由进动(CWFP)序列获得的纵向(T(1))和横向(T(2))弛豫时间信息。对于牛肉中的IMF,CWFP产生的相关系数(r = 0.9)高于传统的 Carr-Purcell-Meiboom-Gill(CPMG)方法(r = -0.25),并且与CPMG一样快速,且脉冲序列更简单。该方法也可应用于其他肉类产品。

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