Li R-Q, Marek A, Smirnov Alex I, Grebel H
Electronic Imaging Center at NJIT and ECE Department, NJIT, Newark, New Jersey 07102, USA.
J Chem Phys. 2008 Sep 7;129(9):095102. doi: 10.1063/1.2972143.
Lipid bilayers are essential structural component of biological membranes of all the living species: from viruses and bacteria to plants and humans. Biophysical and biochemical properties of such membranes are important for understanding physical mechanisms responsible for drug targeting. Binding events between proteins and the membrane may be ascertained by introducing fluorescence markers (chromophores) to the proteins. Here we describe a novel biosensing platform designed to enhance signals of these fluorescence markers. Nanoporous aluminum oxide membranes with and without gold (Au) surface coating have been employed for optical detection of bound conjugated streptavidin to biotinylated lipid bilayers-a model system that mimics protein docking to the membrane surface. Unexpectedly, it was found that fluorescence signals from such structures vary when pumped with E-polarized and H-polarized incident optical beams. The origin of the observed polarization-dependent effects and the implications for enhanced fluorescence detection in a biochip format are being discussed.
从病毒、细菌到植物和人类。此类膜的生物物理和生化特性对于理解药物靶向的物理机制很重要。蛋白质与膜之间的结合事件可以通过向蛋白质中引入荧光标记物（发色团）来确定。在此，我们描述了一种新型生物传感平台，旨在增强这些荧光标记物的信号。有金（Au）表面涂层和无金表面涂层的纳米多孔氧化铝膜已用于光学检测结合到生物素化脂质双层的共轭链霉亲和素——一种模拟蛋白质与膜表面对接的模型系统。出乎意料的是，发现当用E偏振和H偏振入射光束泵浦时，此类结构的荧光信号会发生变化。正在讨论所观察到的偏振依赖性效应的起源以及对生物芯片形式增强荧光检测的影响。
