人类白血病淋巴细胞和卵巢癌中增强的多聚ADP-核糖基化作用。

Enhanced poly ADP-ribosylation in human leukemia lymphocytes and ovarian cancers.

作者信息

Singh N

机构信息

Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.

出版信息

Cancer Lett. 1991 Jun 14;58(1-2):131-5. doi: 10.1016/0304-3835(91)90035-g.

DOI:10.1016/0304-3835(91)90035-g

PMID:1904797

Abstract

Freshly isolated human peripheral blood lymphocytes from leukemia (AML, ALL, CML) subjects, showed a 2.5-3.5-fold increase in the poly ADPR transferase (poly ADPRT) activity whereas ovarian cancers showed a 2-fold increase. This was accompanied by a drop in NAD levels of 45%-63% in leukemia cells and 40% in ovarian cancers. Tumour promoters phorbol-12-myristate-13-acetate (PMA) and mezerein produced an increase in poly ADPRT activity in both normal and CML lymphocytes, but the increase was more marked in the case of normals. This was accompanied by a drop in NAD levels. The results presented show a marked increase in poly ADP-ribosylation in malignant cells but normal lymphocytes showed a greater response to tumour promoters as compared to CML lymphocytes.

摘要

从白血病（急性髓系白血病、急性淋巴细胞白血病、慢性髓系白血病）患者新鲜分离的人外周血淋巴细胞，其多聚腺苷二磷酸核糖转移酶（poly ADPRT）活性增加了2.5至3.5倍，而卵巢癌患者的该酶活性增加了2倍。这伴随着白血病细胞中烟酰胺腺嘌呤二核苷酸（NAD）水平下降45%至63%，卵巢癌细胞中NAD水平下降40%。肿瘤促进剂佛波酯-12-肉豆蔻酸酯-13-乙酸酯（PMA）和密执毒素可使正常淋巴细胞和慢性髓系白血病淋巴细胞的poly ADPRT活性增加，但正常淋巴细胞的增加更为明显。这也伴随着NAD水平的下降。所呈现的结果表明恶性细胞中的多聚ADP核糖基化显著增加，但与慢性髓系白血病淋巴细胞相比，正常淋巴细胞对肿瘤促进剂的反应更大。