Ding Kuke, Yang Chunjie, Shen Jingjing, Xu Lili, Li Yanling, Zhou Pinkun, Zeng Yanjun
Biomedical Engineering School, Capital Medical University, Beijing, 100069, China.
Cell Mol Neurobiol. 2009 May;29(3):383-9. doi: 10.1007/s10571-008-9330-x. Epub 2008 Dec 2.
To investigate the effects of holocarboxylase synthetase (HCS) gene to irradiation in a time- and dose-dependent manner.
AHH-1 cells, Hela cells, and the nude mice inoculated with tumor cells were exposed to gamma-ray of cobalt 60. The mRNA level of HCS was detected using real-time PCR. The profiles of mRNA of HCS after radiation were analyzed and described.
The expression of HCS gene was higher in AHH-1 cells than that in Hela cells. Furthermore, both AHH-1 and Hela displayed similar time-dependent transcriptional levels of HCS gene to radiations at the dose of 2 and 10 Gy. We set the parameters of D, V, R, F, N to quantitatively analyze HCS gene regulation in response to irradiation. We also observed that irradiation resulted in higher levels of HCS in human hepatocyte xenografts, compared with three other types of human tumor xenografts. 2 and 4 Gy radiation had little influence on the HCS gene of human lung cancer and brain cancer, mammary gland cancer was more sensitive to the 4 Gy gamma-ray dose compared with the 2 Gy gamma-ray dose.
HCS is a good radiation-responsive gene. It may be used as a candidate for developing novel biomarkers of radiation damage and it has a great potential to be used in radiation-therapy of liver tumors.
以时间和剂量依赖的方式研究全羧化酶合成酶（HCS）基因对辐射的影响。
将AHH-1细胞、Hela细胞以及接种肿瘤细胞的裸鼠暴露于钴60的γ射线。使用实时PCR检测HCS的mRNA水平。分析并描述辐射后HCS的mRNA谱。
AHH-1细胞中HCS基因的表达高于Hela细胞。此外，在2 Gy和10 Gy剂量的辐射下，AHH-1细胞和Hela细胞中HCS基因的转录水平均呈现出相似的时间依赖性。我们设置了D、V、R、F、N参数来定量分析HCS基因对辐射的响应调控。我们还观察到，与其他三种类型的人肿瘤异种移植相比，辐射导致人肝细胞异种移植中HCS水平更高。2 Gy和4 Gy辐射对人肺癌和脑癌的HCS基因影响较小，与2 Gyγ射线剂量相比，乳腺癌对4 Gyγ射线剂量更敏感。
HCS是一个良好的辐射响应基因。它可能作为开发新型辐射损伤生物标志物的候选基因，并且在肝肿瘤放射治疗中具有巨大的应用潜力。
